Bonamia perspora n. sp. (Haplosporidia), a parasite of the oyster Ostreola equestris, is the first Bonamia species known to produce spores

Ryan B. Carnegie, Eugene M. Burreson, P. Mike Hine, Nancy A. Stokes, Corinne Audemard, Melanie J. Bishop, Charles H. Peterson

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Examination of the oyster Ostreola equestris as a potential reservoir host for a species of Bonamia discovered in Crassostrea ariakensis in North Carolina (NC), USA, revealed a second novel Bonamia sp. Histopathology, electron microscopy, and molecular phylogenetic analysis support the designation of a new parasite species, Bonamia perspora n. sp., which is the first Bonamia species shown to produce a typical haplosporidian spore with an orifice and hinged operculum. Spores were confirmed to be from B. perspora by fluorescent in situ hybridization. Bonamia perspora was found at Morehead City and Wilmington, NC, with an overall prevalence of 1.4% (31/2,144). Uninucleate, plasmodial, and sporogonic stages occurred almost exclusively in connective tissues; uninucleate stages (2-6 μm) were rarely observed in hemocytes. Spores were 4.3-6.4 μm in length. Ultrastructurally, uninucleate, diplokaryotic, and plasmodial stages resembled those of other spore-forming haplosporidians, but few haplosporosomes were present, and plasmodia were small. Spore ornamentation consisted of spore wall-derived, thin, flat ribbons that emerged haphazardly around the spore, and which terminated in what appeared to be four-pronged caps. Number of ribbons per spore ranged from 15 to 30, and their length ranged from 1.0 to 3.4 μm. Parsimony analysis identified B. perspora as a sister species to Bonamia ostreae.

LanguageEnglish
Pages232-245
Number of pages14
JournalJournal of Eukaryotic Microbiology
Volume53
Issue number4
DOIs
Publication statusPublished - Jul 2006
Externally publishedYes

Fingerprint

Haplosporida
Bonamia (Protozoa)
Haplosporidium
Ostreidae
Spores
oysters
Parasites
spores
parasites
Bonamia ostreae
Crassostrea ariakensis
Crassostrea
Hemocytes
disease reservoirs
Plasmodium
fluorescence in situ hybridization
hemocytes
Fluorescence In Situ Hybridization
connective tissues
histopathology

Keywords

  • electron microscopy
  • fluorescent in situ hybridization
  • haplosporidian
  • phylogenetics
  • small subunit ribosomal DNA
  • sporulation
  • ultrastructure

Cite this

Carnegie, Ryan B. ; Burreson, Eugene M. ; Mike Hine, P. ; Stokes, Nancy A. ; Audemard, Corinne ; Bishop, Melanie J. ; Peterson, Charles H. / Bonamia perspora n. sp. (Haplosporidia), a parasite of the oyster Ostreola equestris, is the first Bonamia species known to produce spores. In: Journal of Eukaryotic Microbiology. 2006 ; Vol. 53, No. 4. pp. 232-245.
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abstract = "Examination of the oyster Ostreola equestris as a potential reservoir host for a species of Bonamia discovered in Crassostrea ariakensis in North Carolina (NC), USA, revealed a second novel Bonamia sp. Histopathology, electron microscopy, and molecular phylogenetic analysis support the designation of a new parasite species, Bonamia perspora n. sp., which is the first Bonamia species shown to produce a typical haplosporidian spore with an orifice and hinged operculum. Spores were confirmed to be from B. perspora by fluorescent in situ hybridization. Bonamia perspora was found at Morehead City and Wilmington, NC, with an overall prevalence of 1.4{\%} (31/2,144). Uninucleate, plasmodial, and sporogonic stages occurred almost exclusively in connective tissues; uninucleate stages (2-6 μm) were rarely observed in hemocytes. Spores were 4.3-6.4 μm in length. Ultrastructurally, uninucleate, diplokaryotic, and plasmodial stages resembled those of other spore-forming haplosporidians, but few haplosporosomes were present, and plasmodia were small. Spore ornamentation consisted of spore wall-derived, thin, flat ribbons that emerged haphazardly around the spore, and which terminated in what appeared to be four-pronged caps. Number of ribbons per spore ranged from 15 to 30, and their length ranged from 1.0 to 3.4 μm. Parsimony analysis identified B. perspora as a sister species to Bonamia ostreae.",
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Bonamia perspora n. sp. (Haplosporidia), a parasite of the oyster Ostreola equestris, is the first Bonamia species known to produce spores. / Carnegie, Ryan B.; Burreson, Eugene M.; Mike Hine, P.; Stokes, Nancy A.; Audemard, Corinne; Bishop, Melanie J.; Peterson, Charles H.

In: Journal of Eukaryotic Microbiology, Vol. 53, No. 4, 07.2006, p. 232-245.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Carnegie, Ryan B.

AU - Burreson, Eugene M.

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AU - Stokes, Nancy A.

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AU - Bishop, Melanie J.

AU - Peterson, Charles H.

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