TY - JOUR
T1 - Breakdown and quantitation of the forked termination of replication intermediate of Bacillus subtilis
AU - Hanley, P. J B
AU - Carrigan, C. M.
AU - Rowe, D. B.
AU - Wake, R. G.
PY - 1987/8/5
Y1 - 1987/8/5
N2 - Using a procedure that minimizes shear forces, the BamHI-derived forked termination of replication intermediate of Bacillus subtilis, called band I DNA, can be extracted with little or no accompanying band II DNA. It has been shown that band II DNA is a product of band I breakdown. Nuclease P1-mediated breakdown of the forked band I DNA proceeds in two steps. The first causes the release of one of the arms as band II DNA; in the second step, the remaining arm is cleaved away to yield the free stem. It is concluded that band I represents the primary termination of replication intermediate. A quantitative assessment of the level of band I in DNA from cells of the merodiploid strain, GSY1127, growing at different rates has been made. For cells grown in a minimal medium, at least, the experimentally measured level of band I is of the order (approx. 60%) of that predicted for a complete block to movement of the clockwise fork at the replication terminus, erC.
AB - Using a procedure that minimizes shear forces, the BamHI-derived forked termination of replication intermediate of Bacillus subtilis, called band I DNA, can be extracted with little or no accompanying band II DNA. It has been shown that band II DNA is a product of band I breakdown. Nuclease P1-mediated breakdown of the forked band I DNA proceeds in two steps. The first causes the release of one of the arms as band II DNA; in the second step, the remaining arm is cleaved away to yield the free stem. It is concluded that band I represents the primary termination of replication intermediate. A quantitative assessment of the level of band I in DNA from cells of the merodiploid strain, GSY1127, growing at different rates has been made. For cells grown in a minimal medium, at least, the experimentally measured level of band I is of the order (approx. 60%) of that predicted for a complete block to movement of the clockwise fork at the replication terminus, erC.
UR - http://www.scopus.com/inward/record.url?scp=0023645202&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(87)90043-X
DO - 10.1016/0022-2836(87)90043-X
M3 - Article
C2 - 3119858
AN - SCOPUS:0023645202
SN - 0022-2836
VL - 196
SP - 721
EP - 727
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 3
ER -