Brightness and photostability of emerging red and near-IR fluorescent nanomaterials for bioimaging

Philipp Reineck*, Adam Francis, Antony Orth, Desmond Wai Mo Lau, Reece David Valmont Nixon-Luke, Ishan Das Rastogi, Wan Aizuddin Wan Razali, Nicole Maree Cordina, Lindsay Marie Parker, Varun Kumaraswamy Annayya Sreenivasan, Louise Jennifer Brown, Brant Cameron Gibson

*Corresponding author for this work

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Many novel fluorescent nanomaterials exhibit radically different optical properties compared to organic fluorophores that are still the most extensively used class of fluorophores in biology today. Assessing the practical impact of these optical differences for bioimaging experiments is challenging due to a lack of published quantitative benchmarking data. This study therefore directly and quantitatively compares the brightness and photostability of representatives from seven classes of fluorescent materials in spectroscopy and fluorescence microscopy experiments for the first time. These material classes are: organic dyes, semiconductor quantum dots, fluorescent beads, carbon dots, gold nanoclusters, nanodiamonds, and nanorubies. The relative brightness of each material is determined and the minimum material concentrations required to generate sufficient contrast in a fluorescence microscopy image are assessed. The influence of optical filters used for imaging is also discussed and suitable filter combinations are identified. The photostability of all materials is determined under typical imaging conditions and the number of images that can be acquired is inferred. The results are expected to facilitate the transition of novel fluorescent materials from physics and chemistry into biology laboratories.

Original languageEnglish
Pages (from-to)1549-1557
Number of pages9
JournalAdvanced Optical Materials
Volume4
Issue number10
DOIs
Publication statusPublished - 1 Oct 2016

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Keywords

  • bioimaging
  • brightness
  • fluorescence
  • nanomaterials
  • photostability

Cite this

Reineck, P., Francis, A., Orth, A., Lau, D. W. M., Nixon-Luke, R. D. V., Rastogi, I. D., ... Gibson, B. C. (2016). Brightness and photostability of emerging red and near-IR fluorescent nanomaterials for bioimaging. Advanced Optical Materials, 4(10), 1549-1557. https://doi.org/10.1002/adom.201600212