Bronchial epithelial cell extracellular vesicles ameliorate epithelial–mesenchymal transition in COPD pathogenesis by alleviating M2 macrophage polarization

Shengyang He, Duanni Chen, Mengyun Hu, Li Zhang, Caihong Liu, Daniela Traini, Georges E. Grau, Zhengpeng Zeng, Junjuan Lu, Guanzhi Zhou, Lihua Xie*, Shenghua Sun

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Chronic obstructive pulmonary disease (COPD) is partly characterized as epithelial–mesenchymal transition (EMT)-related airflow limitation. Extracellular vesicles (EVs) play crucial roles in the crosstalk between cells, affecting many diseases including COPD. Up to now, the roles of EVs in COPD are still debated. As we found in this investigation, COPD patients have higher miR-21 level in total serum EVs. EMT occurs in lungs of COPD mice. Furthermore, bronchial epithelial cells (BEAS-2B) could generate EVs with less miR-21 when treated with cigarette smoke extract (CSE), impacting less on the M2-directed macrophage polarization than the control-EVs (PBS-treated) according to EVs miR-21 level. Furthermore, the EMT processes in BEAS-2B cells were enhanced with the M2 macrophages proportion when co-cultured. Collectively, these results demonstrate that CSE-treated BEAS-2B cells could alleviate M2 macrophages polarization by modulated EVs, and eventually relieve the EMT process of BEAS-2B cells themselves under COPD pathogenesis, revealing a novel compensatory role of them in COPD.

Original languageEnglish
Pages (from-to)259-271
Number of pages13
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume18
DOIs
Publication statusPublished - Jun 2019
Externally publishedYes

Keywords

  • COPD
  • Epithelial–mesenchymal transition
  • Exosome
  • Extracellular vesicle
  • M2 macrophages
  • miR-21
  • Small airway remodeling

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