TY - JOUR
T1 - BsmI but not FokI polymorphism of VDR gene is contributed in breast cancer
AU - Shahbazi, Shirin
AU - Alavi, Sara
AU - Majidzadeh-A, Keivan
AU - GhaffarPour, Massoud
AU - Soleimani, Azam
AU - Mahdian, Reza
PY - 2013/3
Y1 - 2013/3
N2 - There is growing evidence of a malignancy-protective role for vitamin D in breast cancer. The effects of vitamin D are mediated via the vitamin D receptor (VDR) which is encoded by VDR gene. Several SNPs on VDR gene has attracted research interest, although the magnitude of the impact of VDR allelic variations on breast cancer has been controversial. In the present study, we focused on the distribution of VDR FokI and BsmI polymorphisms in Iranian breast cancer patients. A case-control study was conducted on 296 samples including 140 breast cancer patients and 156 age matched control women. Restriction fragment length polymorphism (RFLP) analysis was performed for BsmI and FokI genotyping. Randomly selected PCR products were subjected to sequencing to verify the RFLP results. A significantly increased risk of breast cancer was observed with BsmI bb or even Bb genotype (OR 2.39, CI 1.17-4.85 and OR 2.28, CI 1.16-4.47, respectively). Nevertheless, statistically significant association between FokI genotypes and breast cancer risk was not observed. This study lends support for an increased risk of breast cancer associated with the VDR BsmI polymorphism.
AB - There is growing evidence of a malignancy-protective role for vitamin D in breast cancer. The effects of vitamin D are mediated via the vitamin D receptor (VDR) which is encoded by VDR gene. Several SNPs on VDR gene has attracted research interest, although the magnitude of the impact of VDR allelic variations on breast cancer has been controversial. In the present study, we focused on the distribution of VDR FokI and BsmI polymorphisms in Iranian breast cancer patients. A case-control study was conducted on 296 samples including 140 breast cancer patients and 156 age matched control women. Restriction fragment length polymorphism (RFLP) analysis was performed for BsmI and FokI genotyping. Randomly selected PCR products were subjected to sequencing to verify the RFLP results. A significantly increased risk of breast cancer was observed with BsmI bb or even Bb genotype (OR 2.39, CI 1.17-4.85 and OR 2.28, CI 1.16-4.47, respectively). Nevertheless, statistically significant association between FokI genotypes and breast cancer risk was not observed. This study lends support for an increased risk of breast cancer associated with the VDR BsmI polymorphism.
KW - Breast cancer
KW - RFLP
KW - Single nucleotide polymorphism
KW - Vitamin D receptor
UR - http://www.scopus.com/inward/record.url?scp=84880631408&partnerID=8YFLogxK
U2 - 10.1007/s12032-012-0393-7
DO - 10.1007/s12032-012-0393-7
M3 - Article
C2 - 23277283
AN - SCOPUS:84880631408
VL - 30
SP - 1
EP - 6
JO - Medical Oncology
JF - Medical Oncology
SN - 1357-0560
IS - 1
M1 - 393
ER -