CD44 regulates dendrite morphogenesis through src tyrosine kinase-dependent positioning of the Golgi

Anna Skupien; Anna Konopka; PaweI Trzaskoma; Josephine Labus; Adam Gorlewicz; Lukasz Swiech; Matylda Babraj; Hubert Dolezyczek; Izabela Figiel; Evgeni Ponimaskin; Jakub Wlodarczyk; Jacek Jaworski; Grzegorz M Wilczynski; Joanna Dzwonek

doi:10.1242/jcs.154542

CD44 regulates dendrite morphogenesis through src tyrosine kinase-dependent positioning of the Golgi

Anna Skupien, Anna Konopka, PaweI Trzaskoma, Josephine Labus, Adam Gorlewicz, Lukasz Swiech, Matylda Babraj, Hubert Dolezyczek, Izabela Figiel, Evgeni Ponimaskin, Jakub Wlodarczyk, Jacek Jaworski, Grzegorz M Wilczynski, Joanna Dzwonek

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The acquisition of proper dendrite morphology is a crucial aspect of neuronal development towards the formation of a functional network. The role of the extracellular matrix and its cellular receptors in this process has remained enigmatic. We report that the CD44 adhesion molecule, the main hyaluronan receptor, is localized in dendrites and plays a crucial inhibitory role in dendritic tree arborization in vitro and in vivo. This novel function is exerted by the activation of Src tyrosine kinase, leading to the alteration of Golgi morphology. The mechanism operates during normal brain development, but its inhibition might have a protective influence on dendritic trees under toxic conditions, during which the silencing of CD44 expression prevents dendritic shortening induced by glutamate exposure. Overall, our results indicate a novel role for CD44 as an essential regulator of dendritic arbor complexity in both health and disease.

Original language	English
Pages (from-to)	5038-5051
Number of pages	14
Journal	Journal of Cell Science
Volume	127
Issue number	23
DOIs	https://doi.org/10.1242/jcs.154542
Publication status	Published - 1 Dec 2014
Externally published	Yes

Keywords

Animals
Animals, Newborn
Antigens, CD44
Cerebral Cortex
Dendrites
Enzyme Activation
Female
Focal Adhesion Kinase 1
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Glutamic Acid
Golgi Apparatus
HEK293 Cells
HeLa Cells
Hippocampus
Humans
Male
Morphogenesis
Mutation
Neurogenesis
RNA Interference
Rats
Rats, Wistar
Signal Transduction
Transfection
src-Family Kinases
Journal Article
Research Support, Non-U.S. Gov't
Src kinase
Extracellular matrix
Dendritic arborization
Golgi fragmentation
CD44

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10.1242/jcs.154542

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Skupien, A., Konopka, A., Trzaskoma, P., Labus, J., Gorlewicz, A., Swiech, L., Babraj, M., Dolezyczek, H., Figiel, I., Ponimaskin, E., Wlodarczyk, J., Jaworski, J., Wilczynski, G. M., & Dzwonek, J. (2014). CD44 regulates dendrite morphogenesis through src tyrosine kinase-dependent positioning of the Golgi. Journal of Cell Science, 127(23), 5038-5051. https://doi.org/10.1242/jcs.154542

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title = "CD44 regulates dendrite morphogenesis through src tyrosine kinase-dependent positioning of the Golgi",

abstract = "The acquisition of proper dendrite morphology is a crucial aspect of neuronal development towards the formation of a functional network. The role of the extracellular matrix and its cellular receptors in this process has remained enigmatic. We report that the CD44 adhesion molecule, the main hyaluronan receptor, is localized in dendrites and plays a crucial inhibitory role in dendritic tree arborization in vitro and in vivo. This novel function is exerted by the activation of Src tyrosine kinase, leading to the alteration of Golgi morphology. The mechanism operates during normal brain development, but its inhibition might have a protective influence on dendritic trees under toxic conditions, during which the silencing of CD44 expression prevents dendritic shortening induced by glutamate exposure. Overall, our results indicate a novel role for CD44 as an essential regulator of dendritic arbor complexity in both health and disease.",

keywords = "Animals, Animals, Newborn, Antigens, CD44, Cerebral Cortex, Dendrites, Enzyme Activation, Female, Focal Adhesion Kinase 1, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Glutamic Acid, Golgi Apparatus, HEK293 Cells, HeLa Cells, Hippocampus, Humans, Male, Morphogenesis, Mutation, Neurogenesis, RNA Interference, Rats, Rats, Wistar, Signal Transduction, Transfection, src-Family Kinases, Journal Article, Research Support, Non-U.S. Gov't, Src kinase, Extracellular matrix, Dendritic arborization, Golgi fragmentation, CD44",

author = "Anna Skupien and Anna Konopka and PaweI Trzaskoma and Josephine Labus and Adam Gorlewicz and Lukasz Swiech and Matylda Babraj and Hubert Dolezyczek and Izabela Figiel and Evgeni Ponimaskin and Jakub Wlodarczyk and Jacek Jaworski and Wilczynski, {Grzegorz M} and Joanna Dzwonek",

note = "{\textcopyright} 2014. Published by The Company of Biologists Ltd.",

year = "2014",

month = dec,

day = "1",

doi = "10.1242/jcs.154542",

language = "English",

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pages = "5038--5051",

journal = "Journal of Cell Science",

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Skupien, A, Konopka, A, Trzaskoma, P, Labus, J, Gorlewicz, A, Swiech, L, Babraj, M, Dolezyczek, H, Figiel, I, Ponimaskin, E, Wlodarczyk, J, Jaworski, J, Wilczynski, GM & Dzwonek, J 2014, 'CD44 regulates dendrite morphogenesis through src tyrosine kinase-dependent positioning of the Golgi', Journal of Cell Science, vol. 127, no. 23, pp. 5038-5051. https://doi.org/10.1242/jcs.154542

TY - JOUR

T1 - CD44 regulates dendrite morphogenesis through src tyrosine kinase-dependent positioning of the Golgi

AU - Skupien, Anna

AU - Konopka, Anna

AU - Trzaskoma, PaweI

AU - Labus, Josephine

AU - Gorlewicz, Adam

AU - Swiech, Lukasz

AU - Babraj, Matylda

AU - Dolezyczek, Hubert

AU - Figiel, Izabela

AU - Ponimaskin, Evgeni

AU - Wlodarczyk, Jakub

AU - Jaworski, Jacek

AU - Wilczynski, Grzegorz M

AU - Dzwonek, Joanna

PY - 2014/12/1

Y1 - 2014/12/1

N2 - The acquisition of proper dendrite morphology is a crucial aspect of neuronal development towards the formation of a functional network. The role of the extracellular matrix and its cellular receptors in this process has remained enigmatic. We report that the CD44 adhesion molecule, the main hyaluronan receptor, is localized in dendrites and plays a crucial inhibitory role in dendritic tree arborization in vitro and in vivo. This novel function is exerted by the activation of Src tyrosine kinase, leading to the alteration of Golgi morphology. The mechanism operates during normal brain development, but its inhibition might have a protective influence on dendritic trees under toxic conditions, during which the silencing of CD44 expression prevents dendritic shortening induced by glutamate exposure. Overall, our results indicate a novel role for CD44 as an essential regulator of dendritic arbor complexity in both health and disease.

AB - The acquisition of proper dendrite morphology is a crucial aspect of neuronal development towards the formation of a functional network. The role of the extracellular matrix and its cellular receptors in this process has remained enigmatic. We report that the CD44 adhesion molecule, the main hyaluronan receptor, is localized in dendrites and plays a crucial inhibitory role in dendritic tree arborization in vitro and in vivo. This novel function is exerted by the activation of Src tyrosine kinase, leading to the alteration of Golgi morphology. The mechanism operates during normal brain development, but its inhibition might have a protective influence on dendritic trees under toxic conditions, during which the silencing of CD44 expression prevents dendritic shortening induced by glutamate exposure. Overall, our results indicate a novel role for CD44 as an essential regulator of dendritic arbor complexity in both health and disease.

KW - Animals

KW - Animals, Newborn

KW - Antigens, CD44

KW - Cerebral Cortex

KW - Dendrites

KW - Enzyme Activation

KW - Female

KW - Focal Adhesion Kinase 1

KW - Gene Expression Regulation, Developmental

KW - Gene Knockdown Techniques

KW - Glutamic Acid

KW - Golgi Apparatus

KW - HEK293 Cells

KW - HeLa Cells

KW - Hippocampus

KW - Humans

KW - Male

KW - Morphogenesis

KW - Mutation

KW - Neurogenesis

KW - RNA Interference

KW - Rats

KW - Rats, Wistar

KW - Signal Transduction

KW - Transfection

KW - src-Family Kinases

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Src kinase

KW - Extracellular matrix

KW - Dendritic arborization

KW - Golgi fragmentation

KW - CD44

UR - http://www.scopus.com/inward/record.url?scp=84914124935&partnerID=8YFLogxK

U2 - 10.1242/jcs.154542

DO - 10.1242/jcs.154542

M3 - Article

C2 - 25300795

SN - 0021-9533

VL - 127

SP - 5038

EP - 5051

JO - Journal of Cell Science

JF - Journal of Cell Science

IS - 23

ER -

CD44 regulates dendrite morphogenesis through src tyrosine kinase-dependent positioning of the Golgi

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Keywords

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