cDNA cloning and nucleotide sequence of rat muscle-specific enolase (ββ enolase)

Yuka Ohshima, Hiromi Mitsui, Yoichi Takayama, Etsuko Kushiya, Kenji Sakimura, Yasuo Takahashi*

*Corresponding author for this work

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    The nucleotide sequence of rat muscle-specific enolase cDNA was determined by sequencing three cDNA clones encoding this enolase isozyme. The nearly full-length cDNA consists of 13-bp 5′- and 84-bp 3′-noncoding regions and a poly(A) tail in addition to a 1302-bp coding region encoding a polypeptide composed of 434 amino acid residues. The deduced primary structure of this enolase isozyme is about 80% similar to those determined previously for rat neuron-specific and non-neuronal enolase isozymes. Southern blot analysis suggested strongly the existence of a single copy of the muscle-specific enolase gene per haploid genome. The mRNA for this enolase isozyme was detected in rat skeletal muscle on day 1 after birth and its level increased rapidly during 10-30 days after birth without any change in its size (1500 bases).

    Original languageEnglish
    Pages (from-to)425-430
    Number of pages6
    JournalFEBS Letters
    Volume242
    Issue number2
    DOIs
    Publication statusPublished - 2 Jan 1989

    Keywords

    • (Muscle)
    • Amino acid sequence
    • cDNA cloning
    • Enolase
    • mRNA

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    Ohshima, Y., Mitsui, H., Takayama, Y., Kushiya, E., Sakimura, K., & Takahashi, Y. (1989). cDNA cloning and nucleotide sequence of rat muscle-specific enolase (ββ enolase). FEBS Letters, 242(2), 425-430. https://doi.org/10.1016/0014-5793(89)80515-0