cDNA cloning of luteinizing hormone subunits from brushtail possum and red kangaroo

Gavan A. Harrison, Elizabeth M. Deane, Desmond W. Cooper*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Luteinizing hormone (LH) plays an important role in the reproductive cycles of all mammals. There is a large amount of both nucleotide and amino acid sequence data available for LH from eutherian mammals, but little is known about the primary structure of LH in marsupials. We have used consensus PCR primers to generate specific probes for screening pituitary cDNA libraries and report the cloning of the cDNAs encoding the αsubunit of LH (also shared by a number of other glycoprotein hormones) and the LH-specific β-subunit, from the common brushtail possum, Trichosurus vulpecula, and the red kangaroo, Macropus rufus. Southern blotting experiments indicated that both genes are probably present as single copies. Comparison of the deduced marsupial protein sequences with homologous sequences from other vertebrates revealed a high degree of conservation, especially for the α-subunit. These sequences represent the first complete primary structures for a marsupial glycoprotein hormone to have been elucidated.

Original languageEnglish
Pages (from-to)638-642
Number of pages5
JournalMammalian Genome
Volume9
Issue number8
DOIs
Publication statusPublished - 1998

Keywords

  • cDNA library
  • luteinizing hormone
  • southern blotting
  • primary structure
  • reproductive cycle

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