Cell culture-derived extracellular vesicles: considerations for reporting cell culturing parameters

Faezeh Shekari; Faisal J. Alibhai; Hossein Baharvand; Verena Börger; Stefania Bruno; Owen Davies; Bernd Giebel; Mario Gimona; Ghasem Hosseini Salekdeh; Lorena Martin-Jaular; Suresh Mathivanan; Inge Nelissen; Esther Nolte-’t Hoen; Lorraine O'Driscoll; Francesca Perut; Stefano Pluchino; Gabriella Pocsfalvi; Carlos Salomon; Carolina Soekmadji; Simon Staubach; Ana Claudia Torrecilhas; Ganesh Vilas Shelke; Tobias Tertel; Dandan Zhu; Clotilde Théry; Kenneth Witwer; Rienk Nieuwland

doi:10.1002/jex2.115

Cell culture-derived extracellular vesicles: considerations for reporting cell culturing parameters

Faezeh Shekari^*, Faisal J. Alibhai, Hossein Baharvand, Verena Börger, Stefania Bruno, Owen Davies, Bernd Giebel, Mario Gimona, Ghasem Hosseini Salekdeh, Lorena Martin-Jaular, Suresh Mathivanan, Inge Nelissen, Esther Nolte-’t Hoen, Lorraine O'Driscoll, Francesca Perut, Stefano Pluchino, Gabriella Pocsfalvi, Carlos Salomon, Carolina Soekmadji, Simon StaubachAna Claudia Torrecilhas, Ganesh Vilas Shelke, Tobias Tertel, Dandan Zhu, Clotilde Théry, Kenneth Witwer, Rienk Nieuwland

^*Corresponding author for this work

School of Natural Sciences

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

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Abstract

Cell culture-conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM-derived EVs (CCM-EV). The CCM-EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell-specific recommendations may need to be established for non-mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM-EV research.

Original language	English
Article number	e115
Pages (from-to)	1-25
Number of pages	25
Journal	Journal of Extracellular Biology
Volume	2
Issue number	10
Early online date	16 Oct 2023
DOIs	https://doi.org/10.1002/jex2.115
Publication status	Published - Oct 2023

Bibliographical note

Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

cell culture-conditioned medium
cellular therapy
ectosomes
exosomes
extracellular vesicles
in vitro
reproducibility
rigor
standardization

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10.1002/jex2.115

Publisher version (open access)Final published version, 998 KBLicence: CC BY

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Shekari, F., Alibhai, F. J., Baharvand, H., Börger, V., Bruno, S., Davies, O., Giebel, B., Gimona, M., Salekdeh, G. H., Martin-Jaular, L., Mathivanan, S., Nelissen, I., Nolte-’t Hoen, E., O'Driscoll, L., Perut, F., Pluchino, S., Pocsfalvi, G., Salomon, C., Soekmadji, C., ... Nieuwland, R. (2023). Cell culture-derived extracellular vesicles: considerations for reporting cell culturing parameters. Journal of Extracellular Biology, 2(10), 1-25. Article e115. https://doi.org/10.1002/jex2.115

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title = "Cell culture-derived extracellular vesicles: considerations for reporting cell culturing parameters",

abstract = "Cell culture-conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM-derived EVs (CCM-EV). The CCM-EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell-specific recommendations may need to be established for non-mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM-EV research.",

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author = "Faezeh Shekari and Alibhai, \{Faisal J.\} and Hossein Baharvand and Verena B{\"o}rger and Stefania Bruno and Owen Davies and Bernd Giebel and Mario Gimona and Salekdeh, \{Ghasem Hosseini\} and Lorena Martin-Jaular and Suresh Mathivanan and Inge Nelissen and \{Nolte-{\textquoteright}t Hoen\}, Esther and Lorraine O'Driscoll and Francesca Perut and Stefano Pluchino and Gabriella Pocsfalvi and Carlos Salomon and Carolina Soekmadji and Simon Staubach and Torrecilhas, \{Ana Claudia\} and Shelke, \{Ganesh Vilas\} and Tobias Tertel and Dandan Zhu and Clotilde Th{\'e}ry and Kenneth Witwer and Rienk Nieuwland",

note = "Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. ",

year = "2023",

month = oct,

doi = "10.1002/jex2.115",

language = "English",

volume = "2",

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journal = "Journal of Extracellular Biology",

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Shekari, F, Alibhai, FJ, Baharvand, H, Börger, V, Bruno, S, Davies, O, Giebel, B, Gimona, M, Salekdeh, GH, Martin-Jaular, L, Mathivanan, S, Nelissen, I, Nolte-’t Hoen, E, O'Driscoll, L, Perut, F, Pluchino, S, Pocsfalvi, G, Salomon, C, Soekmadji, C, Staubach, S, Torrecilhas, AC, Shelke, GV, Tertel, T, Zhu, D, Théry, C, Witwer, K & Nieuwland, R 2023, 'Cell culture-derived extracellular vesicles: considerations for reporting cell culturing parameters', Journal of Extracellular Biology, vol. 2, no. 10, e115, pp. 1-25. https://doi.org/10.1002/jex2.115

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T2 - considerations for reporting cell culturing parameters

AU - Shekari, Faezeh

AU - Alibhai, Faisal J.

AU - Baharvand, Hossein

AU - Börger, Verena

AU - Bruno, Stefania

AU - Davies, Owen

AU - Giebel, Bernd

AU - Gimona, Mario

AU - Salekdeh, Ghasem Hosseini

AU - Martin-Jaular, Lorena

AU - Mathivanan, Suresh

AU - Nelissen, Inge

AU - Nolte-’t Hoen, Esther

AU - O'Driscoll, Lorraine

AU - Perut, Francesca

AU - Pluchino, Stefano

AU - Pocsfalvi, Gabriella

AU - Salomon, Carlos

AU - Soekmadji, Carolina

AU - Staubach, Simon

AU - Torrecilhas, Ana Claudia

AU - Shelke, Ganesh Vilas

AU - Tertel, Tobias

AU - Zhu, Dandan

AU - Théry, Clotilde

AU - Witwer, Kenneth

AU - Nieuwland, Rienk

N1 - Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2023/10

Y1 - 2023/10

N2 - Cell culture-conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM-derived EVs (CCM-EV). The CCM-EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell-specific recommendations may need to be established for non-mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM-EV research.

AB - Cell culture-conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect the biochemical composition, release and possibly the function of CCM-derived EVs (CCM-EV). The CCM-EV task force of the Rigor and Standardization Subcommittee of the International Society for Extracellular Vesicles aims to identify relevant cell culturing parameters, describe their effects based on current knowledge, recommend reporting parameters and identify outstanding questions. While some recommendations are valid for all cell types, cell-specific recommendations may need to be established for non-mammalian sources, such as bacteria, yeast and plant cells. Current progress towards these goals is summarized in this perspective paper, along with a checklist to facilitate transparent reporting of cell culturing parameters to improve the reproducibility of CCM-EV research.

KW - cell culture-conditioned medium

KW - cellular therapy

KW - ectosomes

KW - exosomes

KW - extracellular vesicles

KW - in vitro

KW - reproducibility

KW - rigor

KW - standardization

UR - https://www.scopus.com/pages/publications/85195829516

U2 - 10.1002/jex2.115

DO - 10.1002/jex2.115

M3 - Article

C2 - 38939735

AN - SCOPUS:85195829516

SN - 2768-2811

VL - 2

SP - 1

EP - 25

JO - Journal of Extracellular Biology

JF - Journal of Extracellular Biology

IS - 10

M1 - e115

ER -

Cell culture-derived extracellular vesicles: considerations for reporting cell culturing parameters

Abstract

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Keywords

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