كاهشبيان پروتئين هاى ميتوكندريايى در پروتئوم سيستم عصبى مركزى مدل حيوانى ب بيمارى مالتيپل اسكلروزيس

Translated title of the contribution: Central nervous system proteomics in animal model of multiple sclerosis revealed down-regulation of mithochondrial proteins

Abolhassan Shahzadeh Fazeli, Mohammad Hossein Sanati, Davood Nasrabadi, Alireza Pouya, Hossein Baharvand, Ghasem Hosseini Salekdeh

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Objective: Detection of central nervous system (CNS) molecular defects in an animal model of multiple sclerosis. 

Materials and Methods: Experimental autoimmune encephalomyelitis (EAE) was induced by a myelin oligodendrocyte glycoprotein. Protein expression profiles in the central nervous system between healthy clinical scores 1 and 3 of EAE were studied using a two dimensional electrophoresis based proteomics approach coupled with MALDI TOF/TOF mass spectrometry. 

Results: We identified 8 mitochondrial proteins that were differentially expressed in CNS, all of them down-regulated in scores 1 and/or 3. Of these, 5 proteins belong to the mitochondrial respiratory chain including: NADH dehydrogenase (ubiquinone) Fe-S protein 8, cytochrome c oxidase Va, cytochrome c oxidase Vb, ATP5B, NADH dehydrogenase (ubiquinone) flavoprotein 2. We also observed down-regulation of three other mitochondrial proteins including: glutaredoxin 5, estradiol 17 beta-dehydrogenase 8 and isocitrate dehydrogenase. 

Conclusion: Down-regulation of mitochondrial proteins supported the hypothesis that hypoxia-like tissue injury in multiple sclerosis (MS) lesions may be due to mitochondrial impairment.

Translated title of the contributionCentral nervous system proteomics in animal model of multiple sclerosis revealed down-regulation of mithochondrial proteins
Original languageArabic
Pages (from-to)236-243
Number of pages8
JournalYakhteh
Volume11
Issue number2
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • mitochondria
  • proteomics
  • EAE
  • multiple sclerosis

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