Abstract
Aim: To determine the distribution of peritubular capillaries in an autosomalrecessive rat model of polycystic kidney disease (PKD).Introduction: In humans with end-stage autosomal dominant PKD, focal capil-lary networks develop in the interstitial compartment surrounding cysts, whereasthe microvasculature in the remainder of the kidney seems to disappear. Incontrast, in autosomal recessive PKD, synchronized and diffuse fusiform cystformation occurs, but the effect (if any) on the distribution of peritubular capil-laries is not known.
Methods: Archival paraffin sections of kidneys from Lewis polycystic kidneydisease (LPK) rats (an autosomal recessive PKD model) were examined at 3, 6, 12,16 and 24 weeks of age and compared to control animals. Peritubular capillarydistribution was assessed by immunostaining using monoclonal antibodiesdirected against either a cell membrane antigen (JG-12) or cell surface antigen(RECA-1) in rat endothelial cells.
Results: Preliminary experiments at the week 12 timepoint have been con-ducted. Paraffin sections fixed in methylcarnoy’s solution provided better immu-noreactivity, for either JG-12 or RECA-1, compared to formalin-fixed sectionsthat underwent microwave antigen retrieval. In control rats, RECA-1 demon-strated uniform and intense staining of the peritubular capillaries. In contrast, inLPK rats, there was an almost complete and diffuse loss of peritubular capillarystaining. No significant staining of the peritubular capillaries was demonstrated ineither control or LPK rats incubated with JG-12. However, both RECA-1 andJG-12 stained glomerular capillaries in the non-diseased and LPK rats.
Conclusion: There was a profound loss of peritubular capillary immunoreactivityin LPK rats at week 12, as assessed by RECA-1 staining. Further investigations toimprove JG-12 antibody staining need to be conducted. Once optimized bothRECA-1 and JG-12 staining can be performed on renal tissue from all time points,in order to determine the evolution of peritubular capillary distribution in thismodel.
Methods: Archival paraffin sections of kidneys from Lewis polycystic kidneydisease (LPK) rats (an autosomal recessive PKD model) were examined at 3, 6, 12,16 and 24 weeks of age and compared to control animals. Peritubular capillarydistribution was assessed by immunostaining using monoclonal antibodiesdirected against either a cell membrane antigen (JG-12) or cell surface antigen(RECA-1) in rat endothelial cells.
Results: Preliminary experiments at the week 12 timepoint have been con-ducted. Paraffin sections fixed in methylcarnoy’s solution provided better immu-noreactivity, for either JG-12 or RECA-1, compared to formalin-fixed sectionsthat underwent microwave antigen retrieval. In control rats, RECA-1 demon-strated uniform and intense staining of the peritubular capillaries. In contrast, inLPK rats, there was an almost complete and diffuse loss of peritubular capillarystaining. No significant staining of the peritubular capillaries was demonstrated ineither control or LPK rats incubated with JG-12. However, both RECA-1 andJG-12 stained glomerular capillaries in the non-diseased and LPK rats.
Conclusion: There was a profound loss of peritubular capillary immunoreactivityin LPK rats at week 12, as assessed by RECA-1 staining. Further investigations toimprove JG-12 antibody staining need to be conducted. Once optimized bothRECA-1 and JG-12 staining can be performed on renal tissue from all time points,in order to determine the evolution of peritubular capillary distribution in thismodel.
| Original language | English |
|---|---|
| Article number | 213 |
| Pages (from-to) | A156-A156 |
| Number of pages | 1 |
| Journal | Nephrology |
| Volume | 13 |
| Issue number | Supplement s3 |
| Publication status | Published - Sept 2008 |
| Externally published | Yes |
| Event | 44 Annual Scientific Meeting of the Australian and New Zealand Society of Nephrology - Newcatle, Australia Duration: 6 Sept 2008 → 10 Sept 2008 |