CHANGES IN THE VISCOSITY OF HYALURONIC ACID AFTER EXPOSURE TO A MYELOPEROXIDASE‐DERIVED OXIDANT

Mark S. Baker, Simon P. Green, Denis A. Lowther*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

Both purified hyaluronic acid (HA) and bovine synovial fluid react with OCl, the major oxidant produced by the myeloperoxidase (MPO)/H2O2/Cl system, resulting in a decrease in their specific viscosity. This reaction is inhibited in the presence of excess methionine. H2O2 alone decreases the viscosity of HA, presumably by the Fenton reaction, in the absence (but not in the presence) of the iron chelator, diethyltriaminepentacetic acid (DETAPAC). In the presence of DETAPAC, incubation of HA with the complete MPO/H2O2/Cl system lowered the viscosity of HA. Analysis of 3H‐HA exposed to OCl by gel filtration chromatography indicated that cleavage of HA occurred only at higher OCl concentrations. We suggest that the reduction in viscosity of HA by the MPO/H2O2/Cl system may be due to a combination of oxidative cleavage and changes in the conformation of the molecule. We speculate that the changes in the molecular size of rheumatoid synovial fluid HA may be due to the action of the neutrophil MPO/H2O2/Cl system.

Original languageEnglish
Pages (from-to)461-467
Number of pages7
JournalArthritis & Rheumatism
Volume32
Issue number4
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Dive into the research topics of 'CHANGES IN THE VISCOSITY OF HYALURONIC ACID AFTER EXPOSURE TO A MYELOPEROXIDASE‐DERIVED OXIDANT'. Together they form a unique fingerprint.

Cite this