Characterisation of sin, a potential recombinase-encoding gene from Staphylococcus aureus

Ian T. Paulsen, Matthew T. Gillespie, Tim G. Littlejohn, Orawadee Hanvivatvong, Sally J. Rowland, Keith G. H. Dyke, Ronald A. Skurray*

*Corresponding author for this work

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The staphylococcal β-lactamase (Bla) transposon Tn4002 has previously been reported to have a high level of insertional specificity for a 1.8-kb region on the plasmid pSK1. Nucleotide sequences of this region and of a related region on plasmid pl9789 were determined. Sequence analysis revealed that these two plasmids contain sin, a gene whose deduced product shows similarity to a family of DNA recombinases. Southern hybridisation analysis indicated that sin is located on α-, β- and γ-families of Bla plasmids and on pSK1 family plasmids. A region of dyad symmetry located upstream from sin on pSK1 and pI9789 was identified as the site of insertions of Tn552 and Tn4002 in separate isolates.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalGene
Volume141
Issue number1
DOIs
Publication statusPublished - 8 Apr 1994
Externally publishedYes

Keywords

  • plasmids
  • Resolvases
  • transposon insertions
  • β-lactamase transposons

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    Paulsen, I. T., Gillespie, M. T., Littlejohn, T. G., Hanvivatvong, O., Rowland, S. J., Dyke, K. G. H., & Skurray, R. A. (1994). Characterisation of sin, a potential recombinase-encoding gene from Staphylococcus aureus. Gene, 141(1), 109-114. https://doi.org/10.1016/0378-1119(94)90136-8