Abstract
The productivity of rice crops is threatened by a number of different environmental stresses. We have investigated the proteomic response of rice varieties and species with different genetic backgrounds, when exposed to a range of different abiotic stresses, including drought, high and low temperatures, and salt. This presentation integrates results of many different rice stress response studies performed in our laboratory over the years.
In one exemplar study, plants from 8 different Oryza sativa varieties, and two other rice species, were subjected to drought stress and recovery. Physiological parameters including leaf water potential, photosynthetic and respiratory performance, and plant growth rates were measured. Proteins from tissues of young rice plants were extracted, peptides were separated using reversed phase nanoLC, and identified and quantified using high resolution orbitrap mass spectrometry, followed by peptide to spectrum matching.
In a second example, rice plants were subject to multiple abiotic stress conditions simultaneously, with or without prior treatment with the stress hormone ABA, and characterised in detail at both the proteomic and transcriptomic level. This allowed us to tease apart the tightly integrated networks of genes and proteins involved, highlighting the role of the TCA cycle and photosynthesis related proteins in complex networks acting in both stress response and ABA signalling.
Our studies on abiotic stress in rice over many years have identified a large number of novel stress response proteins. We have begun the process of functionally characterising some of these, by expressing them in yeast cells and tobacco leaves and screening those for stress response phenotypes. Initial data from that study will be presented here, which shows that homologues of specific uncharacterised genes are able to confer resistance to various stresses in yeast.
In one exemplar study, plants from 8 different Oryza sativa varieties, and two other rice species, were subjected to drought stress and recovery. Physiological parameters including leaf water potential, photosynthetic and respiratory performance, and plant growth rates were measured. Proteins from tissues of young rice plants were extracted, peptides were separated using reversed phase nanoLC, and identified and quantified using high resolution orbitrap mass spectrometry, followed by peptide to spectrum matching.
In a second example, rice plants were subject to multiple abiotic stress conditions simultaneously, with or without prior treatment with the stress hormone ABA, and characterised in detail at both the proteomic and transcriptomic level. This allowed us to tease apart the tightly integrated networks of genes and proteins involved, highlighting the role of the TCA cycle and photosynthesis related proteins in complex networks acting in both stress response and ABA signalling.
Our studies on abiotic stress in rice over many years have identified a large number of novel stress response proteins. We have begun the process of functionally characterising some of these, by expressing them in yeast cells and tobacco leaves and screening those for stress response phenotypes. Initial data from that study will be presented here, which shows that homologues of specific uncharacterised genes are able to confer resistance to various stresses in yeast.
| Original language | English |
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| Publication status | Published - 2024 |
| Event | 6th International and 18th Iranian Genetics Congress, 2024 - Tehran, Iran, Islamic Republic of Duration: 21 May 2024 → 23 May 2024 |
Conference
| Conference | 6th International and 18th Iranian Genetics Congress, 2024 |
|---|---|
| Country/Territory | Iran, Islamic Republic of |
| City | Tehran |
| Period | 21/05/24 → 23/05/24 |