Characterisation of the pressure dependency of pulse wave velocity in the mouse aorta

    Research output: Contribution to journalMeeting abstractpeer-review

    Abstract

    Objectives: Studies in rats have shown that the blood pressure dependent characteristics of arterial stiffness, as measured by pulse wave velocity (PWV), are useful in elucidating arterial properties contributing to hypertension in different disease models. Transgenic mouse models are increasingly being used to investigate underlying pathophysiological mechanisms of arterial stiffness. We have developed techniques in the rat that have been successful in characterising pressure-dependent PWV in the murine aorta in vivo. Methods: Beat-to-beat aortic PWV was measured in isoflurane anesthetized adult mice (n=5) using two high fidelity pressure sensors; one placed in the aortic arch via the left carotid artery, and the second underneath the abdominal aorta, to record extravascular tonometric pressure [1]. PWV was characterised across a range of physiological blood pressures by intravenous bolus injections of phenylephrine (3.0μg) and sodium nitroprusside (3.0μg), to increase and decrease blood pressure respectively. Results: PWV (m/s) increased with MAP (mmHg) across the MAP range of 40-125mmHg according to a relationship described by a second order polynomial: PWV = 0.0006.MAP2- 0.0564.MAP+3.9711 (R2 = 0.98). This indicates that the sensitivity of PWV to changes in MAP increases linearly with pressure. Conclusion: This study provides, for the first time, the pressure-dependent relationship of PWV in the murine aorta. It also lays the groundwork for future experiments in knockout mice which will enables investigations of both altered vascular responses in hypertension and pressure dependent and independent mechanisms of arterial stiffness. References: [1] Wang YX, Halks-Miller M, Vergona R, Sullivan ME, Fitch R, Mallari C, et al. Increased aortic stiffness assessed by pulse wave velocity in apolipoprotein E-deficient mice. American Journal of Physiology - Heart and Circulatory Physiology. 2000;278(2 47- 2):H428-H34.
    Original languageEnglish
    Article number542
    Pages (from-to)e159
    Number of pages1
    JournalJournal of Hypertension
    Volume30
    Issue numbere-Supplement 1
    DOIs
    Publication statusPublished - 2012
    Event24th Meeting of the International Society of Hypertension - Sydney, Australia
    Duration: 30 Sep 20124 Oct 2012

    Fingerprint

    Dive into the research topics of 'Characterisation of the pressure dependency of pulse wave velocity in the mouse aorta'. Together they form a unique fingerprint.

    Cite this