TY - JOUR
T1 - Characterisation of the pvdE gene which is required for pyoverdine synthesis in Pseudomonas aeruginosa
AU - McMorran, B. J.
AU - Merriman, M. E.
AU - Rombel, I. T.
AU - Lamont, I. L.
PY - 1996/10/17
Y1 - 1996/10/17
N2 - Pseudomonas aeruginosa (Pa) strain PAO synthesises a siderophore, pyoverdine (Pvd), when grown under conditions of iron starvation. Pvd consists of a dihydroxyquinoline group attached to an 8-amino-acid-residue peptide. DNA spanning at least 78 kb of the chromosome is required for Pvd synthesis, but to date only three genes involved in this process have been characterised. We report the characterisation of a fourth Pa gene, pvdE, which we show to be required for Pvd synthesis. The deduced amino acid sequence of PvdE indicates that the protein is a member of the ATP-binding-cassette (ABC) family of membrane transporter proteins, and this is the first example of the involvement of an ABC-type protein in siderophore synthesis.
AB - Pseudomonas aeruginosa (Pa) strain PAO synthesises a siderophore, pyoverdine (Pvd), when grown under conditions of iron starvation. Pvd consists of a dihydroxyquinoline group attached to an 8-amino-acid-residue peptide. DNA spanning at least 78 kb of the chromosome is required for Pvd synthesis, but to date only three genes involved in this process have been characterised. We report the characterisation of a fourth Pa gene, pvdE, which we show to be required for Pvd synthesis. The deduced amino acid sequence of PvdE indicates that the protein is a member of the ATP-binding-cassette (ABC) family of membrane transporter proteins, and this is the first example of the involvement of an ABC-type protein in siderophore synthesis.
KW - ABC transporter protein
KW - Fluorescent pseudomonad
KW - Siderophore synthesis
UR - http://www.scopus.com/inward/record.url?scp=0030591786&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(96)00209-0
DO - 10.1016/0378-1119(96)00209-0
M3 - Article
C2 - 8918232
AN - SCOPUS:0030591786
SN - 0378-1119
VL - 176
SP - 55
EP - 59
JO - Gene
JF - Gene
IS - 1-2
ER -