Characteristics of equine herpesvirus 1 glycoproteins expressed in insect cells

J. Millar Whalley*, Daria N. Love, Deepanker Tewari, Hugh J. Field

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A series of recombinant baculoviruses containing genes for glycoproteins C, D, H and L of equine herpesvirus 1 (EHV-1) have been constructed, and the EHV-1 products characterised by gel electrophoresis and immunoblotting. The EHV-1 glycoproteins expressed in insect cells were similar but not identical in apparent sizes to those expressed in EHV-1 infected mammalian cells. Each of the EHV-1 products was recognised by convalescent equine sera, indicating that they were all targets for an equine immune response. Mice immunised with baculovirus-expressed EHV-1 gD and gC acquired an enhanced ability to clear challenge EHV-1 from respiratory tissues, in association with both neutralising antibody and cell mediated immune responses.

Original languageEnglish
Pages (from-to)193-201
Number of pages9
JournalVeterinary Microbiology
Volume46
Issue number1-3
DOIs
Publication statusPublished - 1995

Keywords

  • Equine herpesvirus 1
  • Glycoproteins
  • Immune responses
  • Mouse model
  • Recombinant baculoviruses

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