Abstract
A series of recombinant baculoviruses containing genes for glycoproteins C, D, H and L of equine herpesvirus 1 (EHV-1) have been constructed, and the EHV-1 products characterised by gel electrophoresis and immunoblotting. The EHV-1 glycoproteins expressed in insect cells were similar but not identical in apparent sizes to those expressed in EHV-1 infected mammalian cells. Each of the EHV-1 products was recognised by convalescent equine sera, indicating that they were all targets for an equine immune response. Mice immunised with baculovirus-expressed EHV-1 gD and gC acquired an enhanced ability to clear challenge EHV-1 from respiratory tissues, in association with both neutralising antibody and cell mediated immune responses.
Original language | English |
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Pages (from-to) | 193-201 |
Number of pages | 9 |
Journal | Veterinary Microbiology |
Volume | 46 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - 1995 |
Keywords
- Equine herpesvirus 1
- Glycoproteins
- Immune responses
- Mouse model
- Recombinant baculoviruses