Characteristics of equine herpesvirus 1 glycoproteins expressed in insect cells

J. Millar Whalley; Daria N. Love; Deepanker Tewari; Hugh J. Field

doi:10.1016/0378-1135(95)00083-M

Characteristics of equine herpesvirus 1 glycoproteins expressed in insect cells

J. Millar Whalley^*, Daria N. Love, Deepanker Tewari, Hugh J. Field

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A series of recombinant baculoviruses containing genes for glycoproteins C, D, H and L of equine herpesvirus 1 (EHV-1) have been constructed, and the EHV-1 products characterised by gel electrophoresis and immunoblotting. The EHV-1 glycoproteins expressed in insect cells were similar but not identical in apparent sizes to those expressed in EHV-1 infected mammalian cells. Each of the EHV-1 products was recognised by convalescent equine sera, indicating that they were all targets for an equine immune response. Mice immunised with baculovirus-expressed EHV-1 gD and gC acquired an enhanced ability to clear challenge EHV-1 from respiratory tissues, in association with both neutralising antibody and cell mediated immune responses.

Original language	English
Pages (from-to)	193-201
Number of pages	9
Journal	Veterinary Microbiology
Volume	46
Issue number	1-3
DOIs	https://doi.org/10.1016/0378-1135(95)00083-M
Publication status	Published - 1995

Keywords

Equine herpesvirus 1
Glycoproteins
Immune responses
Mouse model
Recombinant baculoviruses

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10.1016/0378-1135(95)00083-M

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title = "Characteristics of equine herpesvirus 1 glycoproteins expressed in insect cells",

abstract = "A series of recombinant baculoviruses containing genes for glycoproteins C, D, H and L of equine herpesvirus 1 (EHV-1) have been constructed, and the EHV-1 products characterised by gel electrophoresis and immunoblotting. The EHV-1 glycoproteins expressed in insect cells were similar but not identical in apparent sizes to those expressed in EHV-1 infected mammalian cells. Each of the EHV-1 products was recognised by convalescent equine sera, indicating that they were all targets for an equine immune response. Mice immunised with baculovirus-expressed EHV-1 gD and gC acquired an enhanced ability to clear challenge EHV-1 from respiratory tissues, in association with both neutralising antibody and cell mediated immune responses.",

keywords = "Equine herpesvirus 1, Glycoproteins, Immune responses, Mouse model, Recombinant baculoviruses",

author = "\{Millar Whalley\}, J. and Love, \{Daria N.\} and Deepanker Tewari and Field, \{Hugh J.\}",

year = "1995",

doi = "10.1016/0378-1135(95)00083-M",

language = "English",

volume = "46",

pages = "193--201",

journal = "Veterinary Microbiology",

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TY - JOUR

T1 - Characteristics of equine herpesvirus 1 glycoproteins expressed in insect cells

AU - Millar Whalley, J.

AU - Love, Daria N.

AU - Tewari, Deepanker

AU - Field, Hugh J.

PY - 1995

Y1 - 1995

N2 - A series of recombinant baculoviruses containing genes for glycoproteins C, D, H and L of equine herpesvirus 1 (EHV-1) have been constructed, and the EHV-1 products characterised by gel electrophoresis and immunoblotting. The EHV-1 glycoproteins expressed in insect cells were similar but not identical in apparent sizes to those expressed in EHV-1 infected mammalian cells. Each of the EHV-1 products was recognised by convalescent equine sera, indicating that they were all targets for an equine immune response. Mice immunised with baculovirus-expressed EHV-1 gD and gC acquired an enhanced ability to clear challenge EHV-1 from respiratory tissues, in association with both neutralising antibody and cell mediated immune responses.

AB - A series of recombinant baculoviruses containing genes for glycoproteins C, D, H and L of equine herpesvirus 1 (EHV-1) have been constructed, and the EHV-1 products characterised by gel electrophoresis and immunoblotting. The EHV-1 glycoproteins expressed in insect cells were similar but not identical in apparent sizes to those expressed in EHV-1 infected mammalian cells. Each of the EHV-1 products was recognised by convalescent equine sera, indicating that they were all targets for an equine immune response. Mice immunised with baculovirus-expressed EHV-1 gD and gC acquired an enhanced ability to clear challenge EHV-1 from respiratory tissues, in association with both neutralising antibody and cell mediated immune responses.

KW - Equine herpesvirus 1

KW - Glycoproteins

KW - Immune responses

KW - Mouse model

KW - Recombinant baculoviruses

UR - https://www.scopus.com/pages/publications/0029066148

U2 - 10.1016/0378-1135(95)00083-M

DO - 10.1016/0378-1135(95)00083-M

M3 - Article

C2 - 8545956

AN - SCOPUS:0029066148

SN - 0378-1135

VL - 46

SP - 193

EP - 201

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1-3

ER -

Characteristics of equine herpesvirus 1 glycoproteins expressed in insect cells

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Keywords

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