Characterization and pharmacological potential of Lactobacillus sakei 1I1 isolated from fresh water fish Zacco koreanus

Vivek K. Bajpai, Jeong Ho Han, Gyeong Jun Nam, Rajib Majumder, Chanseo Park, Jeongheui Lim*, Woon Kee Paek, Irfan A. Rather, Yong Ha Park

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
13 Downloads (Pure)

Abstract

Background: There are still a large variety of microorganisms among aquatic animals which have not been explored for their pharmacological potential. Hence, present study was aimed to isolate and characterize a potent lactic acid bacterium from fresh water fish sample Zacco koreanus, and to confirm its pharmacological potential. Methods: Isolation of lactic acid bacteria (LAB) from fresh water fish samples was done using serial dilution method. Biochemical identification and molecular characterization of selected LAB isolate 1I1, based on its potent antimicrobial efficacy, was accomplished using API kit and 16S rRNA gene sequencing analysis. Further, 1I1 was assessed for α-glucosidase and tyrosinase inhibitory potential as well as antiviral efficacy against highly pathogenic human influenza virus H1N1 using MDCK cell line in terms of its pharmacological potential. Results: Here, we first time report isolation as well as biochemical and molecular characterization of a lactic acid bacterium Lactobacillus sakei 1I1 isolated from the intestine of a fresh water fish Z. koreanus. As a result, L. sakei 1I1 exhibited potent antimicrobial effect in vitro, and diameter of zones of inhibition of 1I1 against the tested pathogens was found in the range of 13.32 ± 0.51 to 23.16 ± 0.32 mm. Also L. sakei 1I1 at 100 mg/ml exhibited significant (p < 0.05) α-glucosidase and tyrosinase inhibitory activities by 60.69 and 72.59 %, in terms of its anti-diabetic and anti-melanogenic potential, respectively. Moreover, L. sakei 1I1 displayed profound anti-cytopathic effect on MDCK cell line when treated with its ethanol extract (100 mg/ml), confirming its potent anti-viral efficacy against H1N1 influenza virus. Conclusions: These findings reinforce the suggestions that L. sakei 1I1 isolated from the intestine of fresh water fish Z. koreanus might be a candidate of choice for using in pharmacological preparations as an effective drug.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalDARU, Journal of Pharmaceutical Sciences
Volume24
Issue number1
DOIs
Publication statusPublished - 15 Mar 2016
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Anti-tyrosinase activity
  • Anti-viral activity
  • Fish microbiota
  • Lactic acid bacteria
  • α-glucosidase inhibitory activity

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