TY - JOUR
T1 - Characterization of 12 novel microsatellite loci and cross-amplification of four loci in the endangered Australian sea lion (Neophoca cinerea)
AU - Ahonen, Heidi
AU - Lowther, Andrew D.
AU - Goldsworthy, Simon D.
AU - Harcourt, Robert G.
AU - Stow, Adam J.
PY - 2013
Y1 - 2013
N2 - We describe a microsatellite panel of 12 newly developed and four cross-amplification loci for the endangered Australian sea lion, Neophoca cinerea. Next-generation 454 sequencing was used to obtain species-specific partial genomic library. We genotyped 28 individuals sampled from a single breeding colony to characterize these 16 polymorphic loci. Number of alleles per locus ranged from three to seven, and observed and expected heterozygosites from 0. 333 to 0. 852 and from 0. 377 to 0. 787, respectively. These markers will be used to examine genetic diversity, genetic connectivity among colonies and the mating system of Australian sea lions.
AB - We describe a microsatellite panel of 12 newly developed and four cross-amplification loci for the endangered Australian sea lion, Neophoca cinerea. Next-generation 454 sequencing was used to obtain species-specific partial genomic library. We genotyped 28 individuals sampled from a single breeding colony to characterize these 16 polymorphic loci. Number of alleles per locus ranged from three to seven, and observed and expected heterozygosites from 0. 333 to 0. 852 and from 0. 377 to 0. 787, respectively. These markers will be used to examine genetic diversity, genetic connectivity among colonies and the mating system of Australian sea lions.
UR - http://www.scopus.com/inward/record.url?scp=84873689612&partnerID=8YFLogxK
U2 - 10.1007/s12686-012-9788-1
DO - 10.1007/s12686-012-9788-1
M3 - Article
AN - SCOPUS:84873689612
SN - 1877-7252
VL - 5
SP - 283
EP - 285
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 1
ER -