Characterization of 12 polymorphic microsatellite loci in the Port Jackson Shark, Heterodontus portusjacksoni (Meyer, 1793)

Jennalee A. Clark, Culum Brown, Michael R. Gillings, Michael Gardner, Jane E. Williamson, Christopher Izzo, Joanna Day

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The Port Jackson Shark (Heterodontus portusjacksoni) is an epibenthic, oviparous species endemic to the southern half of Australia. Understanding the genetic structure, ecology and reproductive behaviours of this species is essential for developing appropriate management and conservation strategies. However, research has been hampered due a lack of suitable genetic markers. Here, we developed 12 microsatellite loci for H. portusjacksoni. The number of alleles at these polymorphic loci ranged from 3 to 22 and the observed heterozygosity ranged from 0.15 to 0.84. Six of twelve loci successfully cross-amplified in the closely related Crested Hornshark, H. galeatus (Günther, 1870). These markers will aid in studying the spatial and reproductive ecology of H. portusjacksoni and other heterodontid sharks.

LanguageEnglish
Pages505–508
Number of pages4
JournalMarine Biodiversity
Volume49
Issue number1
Early online date2 Aug 2017
DOIs
Publication statusPublished - Feb 2019

Fingerprint

shark
sharks
microsatellite repeats
ecology
loci
reproductive behavior
Galeatus
genetic marker
endemic species
heterozygosity
genetic structure
allele
indigenous species
alleles
genetic markers
Heterodontus portusjacksoni
marker

Keywords

  • elasmobranch
  • cross-amplification
  • population structure
  • dispersal
  • genotype reconstruction
  • nuclear DNA

Cite this

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title = "Characterization of 12 polymorphic microsatellite loci in the Port Jackson Shark, Heterodontus portusjacksoni (Meyer, 1793)",
abstract = "The Port Jackson Shark (Heterodontus portusjacksoni) is an epibenthic, oviparous species endemic to the southern half of Australia. Understanding the genetic structure, ecology and reproductive behaviours of this species is essential for developing appropriate management and conservation strategies. However, research has been hampered due a lack of suitable genetic markers. Here, we developed 12 microsatellite loci for H. portusjacksoni. The number of alleles at these polymorphic loci ranged from 3 to 22 and the observed heterozygosity ranged from 0.15 to 0.84. Six of twelve loci successfully cross-amplified in the closely related Crested Hornshark, H. galeatus (G{\"u}nther, 1870). These markers will aid in studying the spatial and reproductive ecology of H. portusjacksoni and other heterodontid sharks.",
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Characterization of 12 polymorphic microsatellite loci in the Port Jackson Shark, Heterodontus portusjacksoni (Meyer, 1793). / Clark, Jennalee A.; Brown, Culum; Gillings, Michael R.; Gardner, Michael; Williamson, Jane E.; Izzo, Christopher; Day, Joanna.

In: Marine Biodiversity, Vol. 49, No. 1, 02.2019, p. 505–508.

Research output: Contribution to journalArticleResearchpeer-review

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