Characterization of acid-induced molten globule like state of ficin

K. B. Devaraj, Parigi Ramesh Kumar, V. Prakash*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Effect of pH on the conformational behaviour of ficin (EC 3.4.22.3), a cysteine protease from the latex of Ficus carica was monitored by circular dichroism, fluorescence spectroscopy, ANS binding and hydrodynamic studies. The results obtained from near- and far-UV CD, intrinsic fluorescence and ANS binding studies demonstrate that ficin exhibits the characteristic properties of molten globule at acidic conditions between pH 1.4 and 2.0. Ficin at pH 1.4 retained about ∼74% secondary structure with a substantial loss of tertiary structure. The acid-induced state was found to have a compact shape as measured by Stokes radius on size exclusion chromatography.

Original languageEnglish
Pages (from-to)248-254
Number of pages7
JournalJournal of Energy Finance and Development
Volume45
Issue number3
DOIs
Publication statusPublished - 1 Oct 2009
Externally publishedYes

Keywords

  • Acid-unfolding
  • Cysteine protease
  • Ficin
  • Molten globule
  • Protein folding

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