TY - JOUR
T1 - Characterization of O-linked glycosylation motifs in the glycopeptide domain of bovine κ-casein
AU - Pisano, Anthony
AU - Packer, Nicolle H.
AU - Redmond, John W.
AU - Williams, Keith L.
AU - Gooley, Andrew A.
PY - 1994/12
Y1 - 1994/12
N2 - κ-Casein is the major glycoprotein in bovine milk. It has a proteinase-sensitive (chymosin) site which cleaves the glycoprotein into two segments: N-terminal para-κ-casein domain and the C-terminal κ-casein macroglycopeptide domain which is highly heterogeneous in oligosaccharide content. We have identified six sites of O-glycosylation on the macroglycopeptide by solid-phase Edman degradation: Thr121, Thr131, Thr133, Thr136 (A variant only), Thr142 and Thr165. No Ser residues are glycosylated. The glycosylation status of 15 of 17 potential O-glycosylation sites in the B variant was accurately predicted using the four peptide motifs previously proposed for the glycosylation of human glycophorin A (Pisano,A., Redmond,J.W., Williams,K.L. and Gooley,A.A., Glycobiology, 3, 429-435, 1993), provided one additional assumption is made concerning an inhibitory role for a nearby Ile.
AB - κ-Casein is the major glycoprotein in bovine milk. It has a proteinase-sensitive (chymosin) site which cleaves the glycoprotein into two segments: N-terminal para-κ-casein domain and the C-terminal κ-casein macroglycopeptide domain which is highly heterogeneous in oligosaccharide content. We have identified six sites of O-glycosylation on the macroglycopeptide by solid-phase Edman degradation: Thr121, Thr131, Thr133, Thr136 (A variant only), Thr142 and Thr165. No Ser residues are glycosylated. The glycosylation status of 15 of 17 potential O-glycosylation sites in the B variant was accurately predicted using the four peptide motifs previously proposed for the glycosylation of human glycophorin A (Pisano,A., Redmond,J.W., Williams,K.L. and Gooley,A.A., Glycobiology, 3, 429-435, 1993), provided one additional assumption is made concerning an inhibitory role for a nearby Ile.
KW - κ-casein
KW - O-glycosylation motif
KW - Solid-phase Edman degradation
UR - http://www.scopus.com/inward/record.url?scp=0028557973&partnerID=8YFLogxK
U2 - 10.1093/glycob/4.6.837
DO - 10.1093/glycob/4.6.837
M3 - Article
C2 - 7734846
AN - SCOPUS:0028557973
VL - 4
SP - 837
EP - 844
JO - Glycobiology
JF - Glycobiology
SN - 0959-6658
IS - 6
ER -