Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts

R. R. Cordero Otero; J. F. Ubeda Iranzo; A. I. Briones-Perez; N. Potgieter; M. A. Villena; I. S. Pretorius; P. Van Rensburg

Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts

R. R. Cordero Otero, J. F. Ubeda Iranzo, A. I. Briones-Perez, N. Potgieter, M. A. Villena, I. S. Pretorius, P. Van Rensburg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

The β-glucosidase activities of 20 wine-related non-Sac charromyces yeasts were quantified, characterized, and assessed for their efficiency in releasing aroma-enhancing compounds during the winemaking process. Of these enzymatic activities, the β-glucosidase activity of Debaryomyces pseudopolymorphus revealed the most suitable combination of properties in terms of functionality at wine pH, resistance to wine-associated inhibitory compounds (glucose, ethanol, and sulfur dioxide), high subrate affinity, and large aglycone-substrate recognition. Its potential as a wine aroma-enhancing enzyme was confirmed by the significantly increasing concentrations of free volatiles (citronellol, nerol, and geraniol) during the fermentation of Chardonnay juice inoculated with both D. pseudopolymorphus and a widely used commercial starter culture strain Saccharomyces cerevisiae, VIN13.

Original language	English
Pages (from-to)	2564-2569
Number of pages	6
Journal	Journal of Food Science
Volume	68
Issue number	8
Publication status	Published - Sept 2003
Externally published	Yes

Cite this

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title = "Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts",

abstract = "The β-glucosidase activities of 20 wine-related non-Sac charromyces yeasts were quantified, characterized, and assessed for their efficiency in releasing aroma-enhancing compounds during the winemaking process. Of these enzymatic activities, the β-glucosidase activity of Debaryomyces pseudopolymorphus revealed the most suitable combination of properties in terms of functionality at wine pH, resistance to wine-associated inhibitory compounds (glucose, ethanol, and sulfur dioxide), high subrate affinity, and large aglycone-substrate recognition. Its potential as a wine aroma-enhancing enzyme was confirmed by the significantly increasing concentrations of free volatiles (citronellol, nerol, and geraniol) during the fermentation of Chardonnay juice inoculated with both D. pseudopolymorphus and a widely used commercial starter culture strain Saccharomyces cerevisiae, VIN13.",

author = "{Cordero Otero}, {R. R.} and {Ubeda Iranzo}, {J. F.} and Briones-Perez, {A. I.} and N. Potgieter and Villena, {M. A.} and Pretorius, {I. S.} and {Van Rensburg}, P.",

year = "2003",

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language = "English",

volume = "68",

pages = "2564--2569",

journal = "Journal of Food Science",

issn = "0022-1147",

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TY - JOUR

T1 - Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts

AU - Cordero Otero, R. R.

AU - Ubeda Iranzo, J. F.

AU - Briones-Perez, A. I.

AU - Potgieter, N.

AU - Villena, M. A.

AU - Pretorius, I. S.

AU - Van Rensburg, P.

PY - 2003/9

Y1 - 2003/9

N2 - The β-glucosidase activities of 20 wine-related non-Sac charromyces yeasts were quantified, characterized, and assessed for their efficiency in releasing aroma-enhancing compounds during the winemaking process. Of these enzymatic activities, the β-glucosidase activity of Debaryomyces pseudopolymorphus revealed the most suitable combination of properties in terms of functionality at wine pH, resistance to wine-associated inhibitory compounds (glucose, ethanol, and sulfur dioxide), high subrate affinity, and large aglycone-substrate recognition. Its potential as a wine aroma-enhancing enzyme was confirmed by the significantly increasing concentrations of free volatiles (citronellol, nerol, and geraniol) during the fermentation of Chardonnay juice inoculated with both D. pseudopolymorphus and a widely used commercial starter culture strain Saccharomyces cerevisiae, VIN13.

AB - The β-glucosidase activities of 20 wine-related non-Sac charromyces yeasts were quantified, characterized, and assessed for their efficiency in releasing aroma-enhancing compounds during the winemaking process. Of these enzymatic activities, the β-glucosidase activity of Debaryomyces pseudopolymorphus revealed the most suitable combination of properties in terms of functionality at wine pH, resistance to wine-associated inhibitory compounds (glucose, ethanol, and sulfur dioxide), high subrate affinity, and large aglycone-substrate recognition. Its potential as a wine aroma-enhancing enzyme was confirmed by the significantly increasing concentrations of free volatiles (citronellol, nerol, and geraniol) during the fermentation of Chardonnay juice inoculated with both D. pseudopolymorphus and a widely used commercial starter culture strain Saccharomyces cerevisiae, VIN13.

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M3 - Article

AN - SCOPUS:0242410584

SN - 0022-1147

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EP - 2569

JO - Journal of Food Science

JF - Journal of Food Science

IS - 8

ER -

Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts

Abstract

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