Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts

R. R. Cordero Otero; J. F. Ubeda Iranzo; A. I. Briones-Perez; N. Potgieter; M. A. Villena; I. S. Pretorius; P. Van Rensburg

Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts

R. R. Cordero Otero, J. F. Ubeda Iranzo, A. I. Briones-Perez, N. Potgieter, M. A. Villena, I. S. Pretorius, P. Van Rensburg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

The β-glucosidase activities of 20 wine-related non-Sac charromyces yeasts were quantified, characterized, and assessed for their efficiency in releasing aroma-enhancing compounds during the winemaking process. Of these enzymatic activities, the β-glucosidase activity of Debaryomyces pseudopolymorphus revealed the most suitable combination of properties in terms of functionality at wine pH, resistance to wine-associated inhibitory compounds (glucose, ethanol, and sulfur dioxide), high subrate affinity, and large aglycone-substrate recognition. Its potential as a wine aroma-enhancing enzyme was confirmed by the significantly increasing concentrations of free volatiles (citronellol, nerol, and geraniol) during the fermentation of Chardonnay juice inoculated with both D. pseudopolymorphus and a widely used commercial starter culture strain Saccharomyces cerevisiae, VIN13.

Original language	English
Pages (from-to)	2564-2569
Number of pages	6
Journal	Journal of Food Science
Volume	68
Issue number	8
Publication status	Published - Sep 2003
Externally published	Yes

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@article{d77bc3aebafd4713b8022ae9b869c5ed,

title = "Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts",

abstract = "The β-glucosidase activities of 20 wine-related non-Sac charromyces yeasts were quantified, characterized, and assessed for their efficiency in releasing aroma-enhancing compounds during the winemaking process. Of these enzymatic activities, the β-glucosidase activity of Debaryomyces pseudopolymorphus revealed the most suitable combination of properties in terms of functionality at wine pH, resistance to wine-associated inhibitory compounds (glucose, ethanol, and sulfur dioxide), high subrate affinity, and large aglycone-substrate recognition. Its potential as a wine aroma-enhancing enzyme was confirmed by the significantly increasing concentrations of free volatiles (citronellol, nerol, and geraniol) during the fermentation of Chardonnay juice inoculated with both D. pseudopolymorphus and a widely used commercial starter culture strain Saccharomyces cerevisiae, VIN13.",

author = "{Cordero Otero}, {R. R.} and {Ubeda Iranzo}, {J. F.} and Briones-Perez, {A. I.} and N. Potgieter and Villena, {M. A.} and Pretorius, {I. S.} and {Van Rensburg}, P.",

year = "2003",

month = sep,

language = "English",

volume = "68",

pages = "2564--2569",

journal = "Journal of Food Science",

issn = "0022-1147",

publisher = "Wiley-Blackwell, Wiley",

number = "8",

}

Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts. / Cordero Otero, R. R.; Ubeda Iranzo, J. F.; Briones-Perez, A. I.; Potgieter, N.; Villena, M. A.; Pretorius, I. S.; Van Rensburg, P.

In: Journal of Food Science, Vol. 68, No. 8, 09.2003, p. 2564-2569.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterization of the β-Glucosidase Activity Produced by Enological Strains of Non-Saccharomyces Yeasts

AU - Cordero Otero, R. R.

AU - Ubeda Iranzo, J. F.

AU - Briones-Perez, A. I.

AU - Potgieter, N.

AU - Villena, M. A.

AU - Pretorius, I. S.

AU - Van Rensburg, P.

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N2 - The β-glucosidase activities of 20 wine-related non-Sac charromyces yeasts were quantified, characterized, and assessed for their efficiency in releasing aroma-enhancing compounds during the winemaking process. Of these enzymatic activities, the β-glucosidase activity of Debaryomyces pseudopolymorphus revealed the most suitable combination of properties in terms of functionality at wine pH, resistance to wine-associated inhibitory compounds (glucose, ethanol, and sulfur dioxide), high subrate affinity, and large aglycone-substrate recognition. Its potential as a wine aroma-enhancing enzyme was confirmed by the significantly increasing concentrations of free volatiles (citronellol, nerol, and geraniol) during the fermentation of Chardonnay juice inoculated with both D. pseudopolymorphus and a widely used commercial starter culture strain Saccharomyces cerevisiae, VIN13.

AB - The β-glucosidase activities of 20 wine-related non-Sac charromyces yeasts were quantified, characterized, and assessed for their efficiency in releasing aroma-enhancing compounds during the winemaking process. Of these enzymatic activities, the β-glucosidase activity of Debaryomyces pseudopolymorphus revealed the most suitable combination of properties in terms of functionality at wine pH, resistance to wine-associated inhibitory compounds (glucose, ethanol, and sulfur dioxide), high subrate affinity, and large aglycone-substrate recognition. Its potential as a wine aroma-enhancing enzyme was confirmed by the significantly increasing concentrations of free volatiles (citronellol, nerol, and geraniol) during the fermentation of Chardonnay juice inoculated with both D. pseudopolymorphus and a widely used commercial starter culture strain Saccharomyces cerevisiae, VIN13.

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