Characterization of the interaction between heterodimeric αvβ6 integrin and urokinase plasminogen activator receptor (uPAR) using functional proteomics

Seong Beom Ahn, Abidali Mohamedali, Samyuktha Anand, Harish R. Cheruku, Debra Birch, Gopichandran Sowmya, David Cantor, Shoba Ranganathan, David W. Inglis, Ronald Frank, Michael Agrez, Edouard C. Nice, Mark S. Baker

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Urokinase plasminogen activator receptor (uPAR) and the epithelial integrin αvβ6 are thought to individually play critical roles in cancer metastasis. These observations have been highlighted by the recent discovery (by proteomics) of an interaction between these two molecules, which are also both implicated in the epithelial-mesenchymal transition (EMT) that facilitates escape of cells from tissue barriers and is a common signature of cancer metastases. In this study, orthogonal in cellulo and in vitro functional proteomic approaches were used to better characterize the uPAR·αvβ6 interaction. Proximity ligation assays (PLA) confirmed the uPAR·αvβ6 interaction on OVCA429 (ovarian cancer line) and four different colon cancer cell lines including positive controls in cells with de novo β6 subunit expression. PLA studies were then validated using peptide arrays, which also identified potential physical sites of uPAR interaction with αvβ6, as well as verifying interactions with other known uPAR ligands (e.g., uPA, vitronectin) and individual integrin subunits (i.e., αv, β1, β3, and β6 alone). Our data suggest that interaction with uPAR requires expression of the complete αβ heterodimer (e.g., αvβ6), not individual subunits (i.e., αv, β1, β3, or β6). Finally, using in silico structural analyses in concert with these functional proteomics studies, we propose and demonstrate that the most likely unique sites of interaction between αvβ6 and uPAR are located in uPAR domains II and III.

LanguageEnglish
Pages5956-5964
Number of pages9
JournalJournal of Proteome Research
Volume13
Issue number12
DOIs
Publication statusPublished - 5 Dec 2014

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Urokinase Plasminogen Activator Receptors
Integrins
Proteomics
Ligation
Assays
Neoplasm Metastasis
Vitronectin
Epithelial-Mesenchymal Transition
Computer Simulation
Ovarian Neoplasms
Colonic Neoplasms
Neoplasms
Cells
Tissue
Ligands
Cell Line
Peptides
Molecules

Cite this

@article{5d751ae1ceba42079873f23e664b4a9f,
title = "Characterization of the interaction between heterodimeric αvβ6 integrin and urokinase plasminogen activator receptor (uPAR) using functional proteomics",
abstract = "Urokinase plasminogen activator receptor (uPAR) and the epithelial integrin αvβ6 are thought to individually play critical roles in cancer metastasis. These observations have been highlighted by the recent discovery (by proteomics) of an interaction between these two molecules, which are also both implicated in the epithelial-mesenchymal transition (EMT) that facilitates escape of cells from tissue barriers and is a common signature of cancer metastases. In this study, orthogonal in cellulo and in vitro functional proteomic approaches were used to better characterize the uPAR·αvβ6 interaction. Proximity ligation assays (PLA) confirmed the uPAR·αvβ6 interaction on OVCA429 (ovarian cancer line) and four different colon cancer cell lines including positive controls in cells with de novo β6 subunit expression. PLA studies were then validated using peptide arrays, which also identified potential physical sites of uPAR interaction with αvβ6, as well as verifying interactions with other known uPAR ligands (e.g., uPA, vitronectin) and individual integrin subunits (i.e., αv, β1, β3, and β6 alone). Our data suggest that interaction with uPAR requires expression of the complete αβ heterodimer (e.g., αvβ6), not individual subunits (i.e., αv, β1, β3, or β6). Finally, using in silico structural analyses in concert with these functional proteomics studies, we propose and demonstrate that the most likely unique sites of interaction between αvβ6 and uPAR are located in uPAR domains II and III.",
author = "Ahn, {Seong Beom} and Abidali Mohamedali and Samyuktha Anand and Cheruku, {Harish R.} and Debra Birch and Gopichandran Sowmya and David Cantor and Shoba Ranganathan and Inglis, {David W.} and Ronald Frank and Michael Agrez and Nice, {Edouard C.} and Baker, {Mark S.}",
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Characterization of the interaction between heterodimeric αvβ6 integrin and urokinase plasminogen activator receptor (uPAR) using functional proteomics. / Ahn, Seong Beom; Mohamedali, Abidali; Anand, Samyuktha; Cheruku, Harish R.; Birch, Debra; Sowmya, Gopichandran; Cantor, David; Ranganathan, Shoba; Inglis, David W.; Frank, Ronald; Agrez, Michael; Nice, Edouard C.; Baker, Mark S.

In: Journal of Proteome Research, Vol. 13, No. 12, 05.12.2014, p. 5956-5964.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Characterization of the interaction between heterodimeric αvβ6 integrin and urokinase plasminogen activator receptor (uPAR) using functional proteomics

AU - Ahn, Seong Beom

AU - Mohamedali, Abidali

AU - Anand, Samyuktha

AU - Cheruku, Harish R.

AU - Birch, Debra

AU - Sowmya, Gopichandran

AU - Cantor, David

AU - Ranganathan, Shoba

AU - Inglis, David W.

AU - Frank, Ronald

AU - Agrez, Michael

AU - Nice, Edouard C.

AU - Baker, Mark S.

PY - 2014/12/5

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