Characterization of the Kynurenine pathway and quinolinic acid production in macaque macrophages

Chai K. Lim; Margaret M C Yap; Stephen J. Kent; Gabriel Gras; Boubekeur Samah; Jane C. Batten; Robert De Rose; Benjamin Heng; Bruce J. Brew; Gilles J. Guillemin

doi:10.4137/IJTR.S11789

Characterization of the Kynurenine pathway and quinolinic acid production in macaque macrophages

Chai K. Lim, Margaret M C Yap, Stephen J. Kent, Gabriel Gras, Boubekeur Samah, Jane C. Batten, Robert De Rose, Benjamin Heng, Bruce J. Brew, Gilles J. Guillemin^*

^*Corresponding author for this work

Macquarie University Centre for Motor Neuron Disease Research

Research output: Contribution to journal › Article › peer-review

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Abstract

The kynurenine pathway (KP) and one of its end-products, the excitotoxin quinolinic acid (QUIN), are involved in the pathogenesis of several major neuroinflammatory brain diseases. A relevant animal model to study KP metabolism is now needed to assess whether intervention in this pathway may improve the outcome of such diseases. Humans and macaques share a very similar genetic makeup. In this study, we characterized the KP metabolism in macaque primary macrophages of three different species in comparison to human cells. We found that the KP profiles in simian macrophages were very similar to those in humans when challenged with inflammatory cytokines. Further, we found that macaque macrophages are capable of producing a pathophysiological concentration of QUIN. Our data validate the simian model as a relevant model to study the human cellular KP metabolism in the context of inflammation.

Original language	English
Pages (from-to)	7-19
Number of pages	13
Journal	International Journal of Tryptophan Research
Volume	6
DOIs	https://doi.org/10.4137/IJTR.S11789
Publication status	Published - 2013

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Copyright the Author(s), publisher and licensee Libertas Academica Ltd. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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title = "Characterization of the Kynurenine pathway and quinolinic acid production in macaque macrophages",

abstract = "The kynurenine pathway (KP) and one of its end-products, the excitotoxin quinolinic acid (QUIN), are involved in the pathogenesis of several major neuroinflammatory brain diseases. A relevant animal model to study KP metabolism is now needed to assess whether intervention in this pathway may improve the outcome of such diseases. Humans and macaques share a very similar genetic makeup. In this study, we characterized the KP metabolism in macaque primary macrophages of three different species in comparison to human cells. We found that the KP profiles in simian macrophages were very similar to those in humans when challenged with inflammatory cytokines. Further, we found that macaque macrophages are capable of producing a pathophysiological concentration of QUIN. Our data validate the simian model as a relevant model to study the human cellular KP metabolism in the context of inflammation.",

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T1 - Characterization of the Kynurenine pathway and quinolinic acid production in macaque macrophages

AU - Lim, Chai K.

AU - Yap, Margaret M C

AU - Kent, Stephen J.

AU - Gras, Gabriel

AU - Samah, Boubekeur

AU - Batten, Jane C.

AU - De Rose, Robert

AU - Heng, Benjamin

AU - Brew, Bruce J.

AU - Guillemin, Gilles J.

N1 - Copyright the Author(s), publisher and licensee Libertas Academica Ltd. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2013

Y1 - 2013

N2 - The kynurenine pathway (KP) and one of its end-products, the excitotoxin quinolinic acid (QUIN), are involved in the pathogenesis of several major neuroinflammatory brain diseases. A relevant animal model to study KP metabolism is now needed to assess whether intervention in this pathway may improve the outcome of such diseases. Humans and macaques share a very similar genetic makeup. In this study, we characterized the KP metabolism in macaque primary macrophages of three different species in comparison to human cells. We found that the KP profiles in simian macrophages were very similar to those in humans when challenged with inflammatory cytokines. Further, we found that macaque macrophages are capable of producing a pathophysiological concentration of QUIN. Our data validate the simian model as a relevant model to study the human cellular KP metabolism in the context of inflammation.

AB - The kynurenine pathway (KP) and one of its end-products, the excitotoxin quinolinic acid (QUIN), are involved in the pathogenesis of several major neuroinflammatory brain diseases. A relevant animal model to study KP metabolism is now needed to assess whether intervention in this pathway may improve the outcome of such diseases. Humans and macaques share a very similar genetic makeup. In this study, we characterized the KP metabolism in macaque primary macrophages of three different species in comparison to human cells. We found that the KP profiles in simian macrophages were very similar to those in humans when challenged with inflammatory cytokines. Further, we found that macaque macrophages are capable of producing a pathophysiological concentration of QUIN. Our data validate the simian model as a relevant model to study the human cellular KP metabolism in the context of inflammation.

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EP - 19

JO - International Journal of Tryptophan Research

JF - International Journal of Tryptophan Research

ER -

Characterization of the Kynurenine pathway and quinolinic acid production in macaque macrophages

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