Characterization of the xylanolytic enzyme system of the extreme thermophilic anaerobic bacteria Thermotoga maritima, T. neapolitana, and T. thermarum

Anwar Sunna, Jürgen Puls, Garabed Antranikian

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The extreme thermophilic anaerobic bacteria Thermotoga maritima, T. neapolitana, and T. thermarum when grown on xylan produce extremely thermoactive xylanases, β-xylosidases and α-arabinofuranosidases. Most of these enzymes are active over a broad temperature and pH range, namely between 40 and 110°C and pH 4 to 9. The xylanases are active towards soluble, as well as insoluble xylan. Unlike the enzyme system of T. maritima and T. thermarum, the xylanase activity of T. neapolitana is activated (up to 211%) in the presence of Ca2+, Mg2+, Co2+, and Mn2+. The xylanolytic enzyme system of T. thermarum is inactive towards cellulose. Xylan hydrolysis experiments indicate the presence of endoxylanases in the enzyme preparation of all strains investigated, with the main products being xylobiose, xylotriose, and larger xylooligosaccharides.

LanguageEnglish
Pages453-461
Number of pages9
JournalComparative Biochemistry and Physiology - A Physiology
Volume118
Issue number3
DOIs
Publication statusPublished - Nov 1997
Externally publishedYes

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Thermotoga maritima
Anaerobic Bacteria
Xylans
Enzymes
Endo-1,4-beta Xylanases
Cellulose
Hydrolysis
Temperature

Cite this

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title = "Characterization of the xylanolytic enzyme system of the extreme thermophilic anaerobic bacteria Thermotoga maritima, T. neapolitana, and T. thermarum",
abstract = "The extreme thermophilic anaerobic bacteria Thermotoga maritima, T. neapolitana, and T. thermarum when grown on xylan produce extremely thermoactive xylanases, β-xylosidases and α-arabinofuranosidases. Most of these enzymes are active over a broad temperature and pH range, namely between 40 and 110°C and pH 4 to 9. The xylanases are active towards soluble, as well as insoluble xylan. Unlike the enzyme system of T. maritima and T. thermarum, the xylanase activity of T. neapolitana is activated (up to 211{\%}) in the presence of Ca2+, Mg2+, Co2+, and Mn2+. The xylanolytic enzyme system of T. thermarum is inactive towards cellulose. Xylan hydrolysis experiments indicate the presence of endoxylanases in the enzyme preparation of all strains investigated, with the main products being xylobiose, xylotriose, and larger xylooligosaccharides.",
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Characterization of the xylanolytic enzyme system of the extreme thermophilic anaerobic bacteria Thermotoga maritima, T. neapolitana, and T. thermarum. / Sunna, Anwar; Puls, Jürgen; Antranikian, Garabed.

In: Comparative Biochemistry and Physiology - A Physiology, Vol. 118, No. 3, 11.1997, p. 453-461.

Research output: Contribution to journalArticleResearchpeer-review

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