Characterizing liquid turbid media by frequency-domain photon-migration spectroscopy

Biju Cletus, Rainer Künnemeyer, Paul Martinsen, Andrew McGlone, Robert Jordan

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


We present a wavelength-tunable frequency-domain instrument for the characterization of liquid turbid media. The instrument employs a tunable titanium-sapphire laser modulated by an acousto-optic modulator. The absorption and reduced scattering coefficient of Intralipid® 20%, diluted to concentrations of 0.94 to 4.00%, are measured over the wavelength range 710 to 850nm at 10-nm intervals. The standard measurement errors for the absorption and reduced scattering coefficients are 1 and 2.5%, respectively. Extrapolation to 0% Intralipid® concentration gives an absorption coefficient that closely follows that of water, overestimating the absorption of pure water by less than 10%. The reduced scattering coefficient is compared at 750 nm with published results and is found consistent within the experimental error. We compare the reduced scattering coefficient to an estimate based on Mie theory and find the reduced scattering coefficient underestimated the Mie theory result by about 9%.
Original languageEnglish
Pages (from-to)024041-1-024041-7
Number of pages7
JournalJournal of Biomedical Optics
Issue number2
Publication statusPublished - 2009
Externally publishedYes


  • frequency domain spectroscopy
  • photon migration spectroscopy
  • Intralipid
  • absorption
  • scattering
  • reduced scattering


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