Chitosan adhesive for laser tissue repair

A. Lauto*, M. Stoodley, A. Avolio, L. J R Foster

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

1 Citation (Scopus)

Abstract

Background. Laser tissue repair usually relies on haemoderivate solders, based on serum albumin. These solders have intrinsic limitations that impair their widespread use, such as limited repair strength, high solubility, brittleness and viral transmission. Furthermore, the solder activation temperature (65-70°C) can induce significant damage to tissue. In this study, a new laser-activated biomaterial for tissue repair was developed and tested in vitro and in vivo to overcome some of the shortcomings of traditional solders. Materials and Methods. Flexible and insoluble strips of chitosan adhesive (surface area ∼34 mm2, thickness ∼20 μm) were developed and bonded on sheep intestine with a laser fluence and irradiance of 52 ± 2 J/cm2 and ∼15 W/cm2 respectively. The temperature between tissue and adhesive was measured using small thermocouples. The strength of repaired tissue was tested by a calibrated tensiometer. The adhesive was also bonded in vivo to the sciatic nerve of rats to assess the thermal damage induced by the laser (fluence = 65 ± 11 J/cm2, irradiance = 15 W/cm2) four days post-operatively. Results. Chitosan adhesives successfully repaired intestine tissue, achieving a repair strength of 0.50 ± 0.15 N (shear stress = 14.7 ± 4.7 KPa, n=30) at a temperature of 60-65°C. The laser caused demyelination of axons at the operated site; nevertheless, the myelinated axons retained their normal morphology proximally and distally.

Original languageEnglish
Title of host publicationPhotonic Therapeutics and Diagnostics II
Subtitle of host publicationProceedings of SPIE
EditorsNikiforos Kollias, Haishan Zeng, Bernard Choi
Place of PublicationBellingham, WA
PublisherSPIE
Pages1-13
Number of pages13
Volume6078
ISBN (Print)0819461202, 9780819461209
DOIs
Publication statusPublished - 22 Feb 2006
Externally publishedYes
EventPhotonic Therapeutics and Diagnostics II - San Jose, CA, United States
Duration: 21 Jan 200624 Jan 2006

Publication series

NameProgress in Biomedical Optics and Imaging
PublisherSPIE, International Society for Optical Engineering
ISSN (Print)1605-7422

Other

OtherPhotonic Therapeutics and Diagnostics II
CountryUnited States
CitySan Jose, CA
Period21/01/0624/01/06

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