Chlorophyll proteins of the prymnesiophyte Pavlova lutherii (Droop) comb. nov. Identification of the major light-harvesting complex

Roger G. Hiller*, A. W. D. Larkum, Pamela M. Wrench

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

A chlorophyll ac-fucoxanthin light-harvesting protein has been separated by SDS-polyacrylamide gel electrophoresis and by digitonin-sucrose density centrifigation from thylakoids of Pavlova lutherii. It contains a single major polypeptide of 21 kDa, comprises 69% of the total chlorophyll a and is enriched in chlorophyll c compared to the thylakoids. Energy transfer from chlorophyll c and fucoxanthin to chlorophyll a was demonstrated within the protein complex. Antibodies to the 21 kDa apoprotein showed cross-reactivity with the 26-28 kDa apoproteins of higher plant light-harvesting chlorophyll a b protein and with the 19 kDa apoprotein of the light-harvesting complex of diatoms, but much reduced or no cross-reactivity with the major thylakoid polypeptides of dinoflagellates and cryptophytes.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalBBA - Bioenergetics
Volume932
Issue numberC
DOIs
Publication statusPublished - 1988

Keywords

  • (P. lutherii)
  • (Prymnesiophyta)
  • chlorophyll c
  • chlorophyll protein complex
  • fucoxanthin
  • light harvesting complex

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