Chlorophyll proteins of the prymnesiophyte Pavlova lutherii (Droop) comb. nov.: Identification of the major light-harvesting complex

Roger G. Hiller; A. W. D. Larkum; Pamela M. Wrench

doi:10.1016/0005-2728(88)90159-4

Chlorophyll proteins of the prymnesiophyte Pavlova lutherii (Droop) comb. nov. Identification of the major light-harvesting complex

Roger G. Hiller^*, A. W. D. Larkum, Pamela M. Wrench

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A chlorophyll ac-fucoxanthin light-harvesting protein has been separated by SDS-polyacrylamide gel electrophoresis and by digitonin-sucrose density centrifigation from thylakoids of Pavlova lutherii. It contains a single major polypeptide of 21 kDa, comprises 69% of the total chlorophyll a and is enriched in chlorophyll c compared to the thylakoids. Energy transfer from chlorophyll c and fucoxanthin to chlorophyll a was demonstrated within the protein complex. Antibodies to the 21 kDa apoprotein showed cross-reactivity with the 26-28 kDa apoproteins of higher plant light-harvesting chlorophyll a b protein and with the 19 kDa apoprotein of the light-harvesting complex of diatoms, but much reduced or no cross-reactivity with the major thylakoid polypeptides of dinoflagellates and cryptophytes.

Original language	English
Pages (from-to)	223-231
Number of pages	9
Journal	BBA - Bioenergetics
Volume	932
Issue number	C
DOIs	https://doi.org/10.1016/0005-2728(88)90159-4
Publication status	Published - 1988

Keywords

(P. lutherii)
(Prymnesiophyta)
chlorophyll c
chlorophyll protein complex
fucoxanthin
light harvesting complex

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10.1016/0005-2728(88)90159-4

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title = "Chlorophyll proteins of the prymnesiophyte Pavlova lutherii (Droop) comb. nov.: Identification of the major light-harvesting complex",

abstract = "A chlorophyll ac-fucoxanthin light-harvesting protein has been separated by SDS-polyacrylamide gel electrophoresis and by digitonin-sucrose density centrifigation from thylakoids of Pavlova lutherii. It contains a single major polypeptide of 21 kDa, comprises 69% of the total chlorophyll a and is enriched in chlorophyll c compared to the thylakoids. Energy transfer from chlorophyll c and fucoxanthin to chlorophyll a was demonstrated within the protein complex. Antibodies to the 21 kDa apoprotein showed cross-reactivity with the 26-28 kDa apoproteins of higher plant light-harvesting chlorophyll a b protein and with the 19 kDa apoprotein of the light-harvesting complex of diatoms, but much reduced or no cross-reactivity with the major thylakoid polypeptides of dinoflagellates and cryptophytes.",

keywords = "(P. lutherii), (Prymnesiophyta), chlorophyll c, chlorophyll protein complex, fucoxanthin, light harvesting complex",

author = "Hiller, {Roger G.} and Larkum, {A. W. D.} and Wrench, {Pamela M.}",

year = "1988",

doi = "10.1016/0005-2728(88)90159-4",

language = "English",

volume = "932",

pages = "223--231",

journal = "Biochimica et Biophysica Acta - Bioenergetics",

issn = "0005-2728",

publisher = "Elsevier",

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TY - JOUR

T1 - Chlorophyll proteins of the prymnesiophyte Pavlova lutherii (Droop) comb. nov.

T2 - Identification of the major light-harvesting complex

AU - Hiller, Roger G.

AU - Larkum, A. W. D.

AU - Wrench, Pamela M.

PY - 1988

Y1 - 1988

N2 - A chlorophyll ac-fucoxanthin light-harvesting protein has been separated by SDS-polyacrylamide gel electrophoresis and by digitonin-sucrose density centrifigation from thylakoids of Pavlova lutherii. It contains a single major polypeptide of 21 kDa, comprises 69% of the total chlorophyll a and is enriched in chlorophyll c compared to the thylakoids. Energy transfer from chlorophyll c and fucoxanthin to chlorophyll a was demonstrated within the protein complex. Antibodies to the 21 kDa apoprotein showed cross-reactivity with the 26-28 kDa apoproteins of higher plant light-harvesting chlorophyll a b protein and with the 19 kDa apoprotein of the light-harvesting complex of diatoms, but much reduced or no cross-reactivity with the major thylakoid polypeptides of dinoflagellates and cryptophytes.

AB - A chlorophyll ac-fucoxanthin light-harvesting protein has been separated by SDS-polyacrylamide gel electrophoresis and by digitonin-sucrose density centrifigation from thylakoids of Pavlova lutherii. It contains a single major polypeptide of 21 kDa, comprises 69% of the total chlorophyll a and is enriched in chlorophyll c compared to the thylakoids. Energy transfer from chlorophyll c and fucoxanthin to chlorophyll a was demonstrated within the protein complex. Antibodies to the 21 kDa apoprotein showed cross-reactivity with the 26-28 kDa apoproteins of higher plant light-harvesting chlorophyll a b protein and with the 19 kDa apoprotein of the light-harvesting complex of diatoms, but much reduced or no cross-reactivity with the major thylakoid polypeptides of dinoflagellates and cryptophytes.

KW - (P. lutherii)

KW - (Prymnesiophyta)

KW - chlorophyll c

KW - chlorophyll protein complex

KW - fucoxanthin

KW - light harvesting complex

UR - http://www.scopus.com/inward/record.url?scp=0005139736&partnerID=8YFLogxK

U2 - 10.1016/0005-2728(88)90159-4

DO - 10.1016/0005-2728(88)90159-4

M3 - Article

AN - SCOPUS:0005139736

VL - 932

SP - 223

EP - 231

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - C

ER -

Chlorophyll proteins of the prymnesiophyte Pavlova lutherii (Droop) comb. nov. Identification of the major light-harvesting complex

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Keywords

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