Chromatography and generation of specific antisera to synthetic peptides from a protective Boophilus microplus antigen

Phillip J. Sharp*, Bernie V. McInerney, Donald R. J. Smith, Ian F. Turnbull, David H. Kemp, Keith N. Rand, Gary S. Cobon

*Corresponding author for this work

Research output: Contribution to journalConference paper

1 Citation (Scopus)

Abstract

Four oligopeptides corresponding to predicted antigenic regions of the protective Bm86 glycoprotein of the cattle tick Boophilus microplus were synthesized and purified. Three were conjugated to carrier proteins and antisera raised in rabbits and cows. All elicited antipeptide antibodies that recognized Bm86 and recombinant derived products in Western blots; however, only one produced antiserum capable of recognizing native Bm86 in an indirect immunofluorescence assay. Ticks fed in vitro on this antiserum showed no obvious gut damage.

Original languageEnglish
Pages (from-to)189-202
Number of pages14
JournalJournal of Chromatography A
Volume512
DOIs
Publication statusPublished - 20 Jul 1990
Externally publishedYes
EventInternational symposium on high-performance liquid chromatography of proteins, peptides and polynucleotides (9th : 1989) - Philadelphia, United States
Duration: 6 Nov 19898 Nov 1989

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