Chromatography and generation of specific antisera to synthetic peptides from a protective Boophilus microplus antigen

Phillip J. Sharp; Bernie V. McInerney; Donald R. J. Smith; Ian F. Turnbull; David H. Kemp; Keith N. Rand; Gary S. Cobon

doi:10.1016/S0021-9673(01)89485-1

Chromatography and generation of specific antisera to synthetic peptides from a protective Boophilus microplus antigen

Phillip J. Sharp^*, Bernie V. McInerney, Donald R. J. Smith, Ian F. Turnbull, David H. Kemp, Keith N. Rand, Gary S. Cobon

^*Corresponding author for this work

Research output: Contribution to journal › Conference paper › peer-review

2 Citations (Scopus)

Abstract

Four oligopeptides corresponding to predicted antigenic regions of the protective Bm86 glycoprotein of the cattle tick Boophilus microplus were synthesized and purified. Three were conjugated to carrier proteins and antisera raised in rabbits and cows. All elicited antipeptide antibodies that recognized Bm86 and recombinant derived products in Western blots; however, only one produced antiserum capable of recognizing native Bm86 in an indirect immunofluorescence assay. Ticks fed in vitro on this antiserum showed no obvious gut damage.

Original language	English
Pages (from-to)	189-202
Number of pages	14
Journal	Journal of Chromatography A
Volume	512
DOIs	https://doi.org/10.1016/S0021-9673(01)89485-1
Publication status	Published - 20 Jul 1990
Externally published	Yes
Event	International symposium on high-performance liquid chromatography of proteins, peptides and polynucleotides (9th : 1989) - Philadelphia, United States Duration: 6 Nov 1989 → 8 Nov 1989

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10.1016/S0021-9673(01)89485-1

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title = "Chromatography and generation of specific antisera to synthetic peptides from a protective Boophilus microplus antigen",

abstract = "Four oligopeptides corresponding to predicted antigenic regions of the protective Bm86 glycoprotein of the cattle tick Boophilus microplus were synthesized and purified. Three were conjugated to carrier proteins and antisera raised in rabbits and cows. All elicited antipeptide antibodies that recognized Bm86 and recombinant derived products in Western blots; however, only one produced antiserum capable of recognizing native Bm86 in an indirect immunofluorescence assay. Ticks fed in vitro on this antiserum showed no obvious gut damage.",

author = "Sharp, {Phillip J.} and McInerney, {Bernie V.} and Smith, {Donald R. J.} and Turnbull, {Ian F.} and Kemp, {David H.} and Rand, {Keith N.} and Cobon, {Gary S.}",

year = "1990",

month = jul,

day = "20",

doi = "10.1016/S0021-9673(01)89485-1",

language = "English",

volume = "512",

pages = "189--202",

journal = "Journal of Chromatography A",

issn = "0021-9673",

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note = "International symposium on high-performance liquid chromatography of proteins, peptides and polynucleotides (9th : 1989) ; Conference date: 06-11-1989 Through 08-11-1989",

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T1 - Chromatography and generation of specific antisera to synthetic peptides from a protective Boophilus microplus antigen

AU - Sharp, Phillip J.

AU - McInerney, Bernie V.

AU - Smith, Donald R. J.

AU - Turnbull, Ian F.

AU - Kemp, David H.

AU - Rand, Keith N.

AU - Cobon, Gary S.

PY - 1990/7/20

Y1 - 1990/7/20

N2 - Four oligopeptides corresponding to predicted antigenic regions of the protective Bm86 glycoprotein of the cattle tick Boophilus microplus were synthesized and purified. Three were conjugated to carrier proteins and antisera raised in rabbits and cows. All elicited antipeptide antibodies that recognized Bm86 and recombinant derived products in Western blots; however, only one produced antiserum capable of recognizing native Bm86 in an indirect immunofluorescence assay. Ticks fed in vitro on this antiserum showed no obvious gut damage.

AB - Four oligopeptides corresponding to predicted antigenic regions of the protective Bm86 glycoprotein of the cattle tick Boophilus microplus were synthesized and purified. Three were conjugated to carrier proteins and antisera raised in rabbits and cows. All elicited antipeptide antibodies that recognized Bm86 and recombinant derived products in Western blots; however, only one produced antiserum capable of recognizing native Bm86 in an indirect immunofluorescence assay. Ticks fed in vitro on this antiserum showed no obvious gut damage.

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U2 - 10.1016/S0021-9673(01)89485-1

DO - 10.1016/S0021-9673(01)89485-1

M3 - Conference paper

C2 - 2229227

AN - SCOPUS:0025711378

SN - 0021-9673

VL - 512

SP - 189

EP - 202

JO - Journal of Chromatography A

JF - Journal of Chromatography A

T2 - International symposium on high-performance liquid chromatography of proteins, peptides and polynucleotides (9th : 1989)

Y2 - 6 November 1989 through 8 November 1989

ER -

Chromatography and generation of specific antisera to synthetic peptides from a protective Boophilus microplus antigen

Abstract

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