Four oligopeptides corresponding to predicted antigenic regions of the protective Bm86 glycoprotein of the cattle tick Boophilus microplus were synthesized and purified. Three were conjugated to carrier proteins and antisera raised in rabbits and cows. All elicited antipeptide antibodies that recognized Bm86 and recombinant derived products in Western blots; however, only one produced antiserum capable of recognizing native Bm86 in an indirect immunofluorescence assay. Ticks fed in vitro on this antiserum showed no obvious gut damage.
|Number of pages||14|
|Journal||Journal of Chromatography A|
|Publication status||Published - 20 Jul 1990|
|Event||International symposium on high-performance liquid chromatography of proteins, peptides and polynucleotides (9th : 1989) - Philadelphia, United States|
Duration: 6 Nov 1989 → 8 Nov 1989