Class 1 integron containing a new gene cassette, aadA10, associated with Tn1404 from R151

Sally R. Partridge, Christina M. Collis, Ruth M. Hall

    Research output: Contribution to journalArticlepeer-review

    45 Citations (Scopus)


    The carbenicillin, gentamicin, kanamycin, streptomycin, spectinomycin, sulfonamide, and tobramycin resistance determinants found on Pseudomonas aeruginosa plasmid R151 have previously been shown to translocate to another plasmid, R388, and it was inferred that a transposon, Tn1404, carried the resistance determinants. Sequencing of the cassette array from the plasmid known as R388::Tn1404 revealed two known gene cassettes, oxa10 and aadB, and a previously unidentified cassette determining resistance to streptomycin and spectinomycin, here designated aadA10, in the order oxa10-aadB-aadA10. These cassettes replaced the dfrB2-orfA cassette array of R388, indicating that movement of the resistance determinants from R151 to R388 resulted from recombinational exchange between two class 1 integrons rather than transposition. The AadA10 protein is most closely related to AadA6 (85% identical) and AadA7 (80% identical). The aadA10 cassette found here has only a simple site containing a 7-bp spacer derived from attI1 in place of a 59-base element and is likely to represent a derivative of the complete cassette. IntI1-mediated deletion of the aadA10 cassette was not detected, indicating that this single simple site is either inactive or only weakly active.
    Original languageEnglish
    Pages (from-to)2400-2408
    Number of pages9
    JournalAntimicrobial Agents and Chemotherapy
    Issue number8
    Publication statusPublished - 2002


    Dive into the research topics of 'Class 1 integron containing a new gene cassette, aadA10, associated with Tn1404 from R151'. Together they form a unique fingerprint.

    Cite this