Clinical stem-cell sources contain CD8+CD3+ T-cell receptor-negative cells that facilitate bone marrow repopulation with hematopoietic stem cells

Stephanie Bridenbaugh, Linda Kenins, Emilie Bouliong-Pillai, Christian P. Kalberer, Elena Shklovskaya, Alois Gratwohl, Aleksandra Wodnar-Filipowicz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Clinical observations in patients undergoing bone marrow transplantation implicate the involvement of CD8+ cells in promoting the stem-cell engraftment process. These findings are supported by mouse transplant studies, which attributed the engraftment-facilitating function to subpopulations of murine CD8+ cells, but the analogous cells in humans have not been identified. Here, we report that clinical stem-cell grafts contain a population of CD8α+CD3ε+ T-cell receptor-negative cells with an engraftment facilitating function, named candidate facilitating cells (cFCs). Purified cFC augmented human hematopoiesis in NOD/SCID mice receiving suboptimal doses of human CD34+ cells. In vitro, cFCs cocultured with CD34+ cells increased hematopoietic colony formation, suggesting a direct effect on clonogenic precursors. These results provide evidence for the existence of rare human CD8+CD3+TCR- cells with engraftment facilitating properties, the adoptive transfer of which could improve the therapeutic outcome of stemcell transplantation.

Original languageEnglish
Pages (from-to)1735-1738
Number of pages4
JournalBlood
Volume111
Issue number3
DOIs
Publication statusPublished - 2008
Externally publishedYes

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