TY - JOUR
T1 - Clinical stem-cell sources contain CD8+CD3+ T-cell receptor-negative cells that facilitate bone marrow repopulation with hematopoietic stem cells
AU - Bridenbaugh, Stephanie
AU - Kenins, Linda
AU - Bouliong-Pillai, Emilie
AU - Kalberer, Christian P.
AU - Shklovskaya, Elena
AU - Gratwohl, Alois
AU - Wodnar-Filipowicz, Aleksandra
PY - 2008
Y1 - 2008
N2 - Clinical observations in patients undergoing bone marrow transplantation implicate the involvement of CD8+ cells in promoting the stem-cell engraftment process. These findings are supported by mouse transplant studies, which attributed the engraftment-facilitating function to subpopulations of murine CD8+ cells, but the analogous cells in humans have not been identified. Here, we report that clinical stem-cell grafts contain a population of CD8α+CD3ε+ T-cell receptor-negative cells with an engraftment facilitating function, named candidate facilitating cells (cFCs). Purified cFC augmented human hematopoiesis in NOD/SCID mice receiving suboptimal doses of human CD34+ cells. In vitro, cFCs cocultured with CD34+ cells increased hematopoietic colony formation, suggesting a direct effect on clonogenic precursors. These results provide evidence for the existence of rare human CD8+CD3+TCR- cells with engraftment facilitating properties, the adoptive transfer of which could improve the therapeutic outcome of stemcell transplantation.
AB - Clinical observations in patients undergoing bone marrow transplantation implicate the involvement of CD8+ cells in promoting the stem-cell engraftment process. These findings are supported by mouse transplant studies, which attributed the engraftment-facilitating function to subpopulations of murine CD8+ cells, but the analogous cells in humans have not been identified. Here, we report that clinical stem-cell grafts contain a population of CD8α+CD3ε+ T-cell receptor-negative cells with an engraftment facilitating function, named candidate facilitating cells (cFCs). Purified cFC augmented human hematopoiesis in NOD/SCID mice receiving suboptimal doses of human CD34+ cells. In vitro, cFCs cocultured with CD34+ cells increased hematopoietic colony formation, suggesting a direct effect on clonogenic precursors. These results provide evidence for the existence of rare human CD8+CD3+TCR- cells with engraftment facilitating properties, the adoptive transfer of which could improve the therapeutic outcome of stemcell transplantation.
UR - http://www.scopus.com/inward/record.url?scp=38949160052&partnerID=8YFLogxK
U2 - 10.1182/blood-2007-02-076000
DO - 10.1182/blood-2007-02-076000
M3 - Article
C2 - 17984314
AN - SCOPUS:38949160052
SN - 0006-4971
VL - 111
SP - 1735
EP - 1738
JO - Blood
JF - Blood
IS - 3
ER -