Cloning and expression of the Clostridium thermosulfurogenes d-xylose isomerase gene (xylA) in Saccharomyces cerevlsiae

Catharine J. Moes, Isak S. Pretorius, Willem H. Van Zyl*

*Corresponding author for this work

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

A recombinant strain of Saccharomyces cerevisiae containing the bacterial D-xylose isomerase (xylA) gene of Clostridium thermosulfurogenes was constructed. The xylA gene has high thermal stability, functions in an anaerobic environment and has a G+C content of 38.9%, which should render it a good candidate for successful expression in S. cerevisiae. Efficient transcription of the xylA gene under the control of the ADH2 gene promoter and terminator sequences in a recombinant S. cerevisiae strain was shown through Northern blot analysis. However, the xylA recombinant strain was unable to grow on D-xylose as sole carbon source.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalBiotechnology Letters
Volume18
Issue number3
Publication statusPublished - 1996
Externally publishedYes

    Fingerprint

Cite this