Cloning and sequence of IS1000, a putative insertion sequence fromThermus thermophilus HB8

M. K. Ashby, P. L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The complete nucleotide sequences of two copies of a putative insertion sequence IS1000 fromThermus thermophilus HB8 are presented. IS1000 is 1196 base pairs long, contains a long open reading frame which could code for a protein of 317 amino acids, and has imperfect terminal inverted repeats of 6 base pairs (confirmed by the terminal sequencing of 4.5 copies of IS1000), but does not cause a target site duplication. There are at least 6 copies of IS1000 in the genome ofT. thermophilus HB8. A search of the GEN-EMBL data base revealed that the putative 317 ammo acid protein had significant homology with open reading frames in the transposable elements IS110 ofStreptomyces coelicolor and IS492 ofPseudomonas atlantica.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalPlasmid
Volume24
Issue number1
DOIs
Publication statusPublished - 1990
Externally publishedYes

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