Cloning and sequence of IS1000, a putative insertion sequence fromThermus thermophilus HB8

M. K. Ashby; P. L. Bergquist

doi:10.1016/0147-619X(90)90020-D

Cloning and sequence of IS1000, a putative insertion sequence fromThermus thermophilus HB8

M. K. Ashby, P. L. Bergquist^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The complete nucleotide sequences of two copies of a putative insertion sequence IS1000 fromThermus thermophilus HB8 are presented. IS1000 is 1196 base pairs long, contains a long open reading frame which could code for a protein of 317 amino acids, and has imperfect terminal inverted repeats of 6 base pairs (confirmed by the terminal sequencing of 4.5 copies of IS1000), but does not cause a target site duplication. There are at least 6 copies of IS1000 in the genome ofT. thermophilus HB8. A search of the GEN-EMBL data base revealed that the putative 317 ammo acid protein had significant homology with open reading frames in the transposable elements IS110 ofStreptomyces coelicolor and IS492 ofPseudomonas atlantica.

Original language	English
Pages (from-to)	1-11
Number of pages	11
Journal	Plasmid
Volume	24
Issue number	1
DOIs	https://doi.org/10.1016/0147-619X(90)90020-D
Publication status	Published - 1990
Externally published	Yes

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10.1016/0147-619X(90)90020-D

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title = "Cloning and sequence of IS1000, a putative insertion sequence fromThermus thermophilus HB8",

abstract = "The complete nucleotide sequences of two copies of a putative insertion sequence IS1000 fromThermus thermophilus HB8 are presented. IS1000 is 1196 base pairs long, contains a long open reading frame which could code for a protein of 317 amino acids, and has imperfect terminal inverted repeats of 6 base pairs (confirmed by the terminal sequencing of 4.5 copies of IS1000), but does not cause a target site duplication. There are at least 6 copies of IS1000 in the genome ofT. thermophilus HB8. A search of the GEN-EMBL data base revealed that the putative 317 ammo acid protein had significant homology with open reading frames in the transposable elements IS110 ofStreptomyces coelicolor and IS492 ofPseudomonas atlantica.",

author = "Ashby, {M. K.} and Bergquist, {P. L.}",

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doi = "10.1016/0147-619X(90)90020-D",

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T1 - Cloning and sequence of IS1000, a putative insertion sequence fromThermus thermophilus HB8

AU - Ashby, M. K.

AU - Bergquist, P. L.

PY - 1990

Y1 - 1990

N2 - The complete nucleotide sequences of two copies of a putative insertion sequence IS1000 fromThermus thermophilus HB8 are presented. IS1000 is 1196 base pairs long, contains a long open reading frame which could code for a protein of 317 amino acids, and has imperfect terminal inverted repeats of 6 base pairs (confirmed by the terminal sequencing of 4.5 copies of IS1000), but does not cause a target site duplication. There are at least 6 copies of IS1000 in the genome ofT. thermophilus HB8. A search of the GEN-EMBL data base revealed that the putative 317 ammo acid protein had significant homology with open reading frames in the transposable elements IS110 ofStreptomyces coelicolor and IS492 ofPseudomonas atlantica.

AB - The complete nucleotide sequences of two copies of a putative insertion sequence IS1000 fromThermus thermophilus HB8 are presented. IS1000 is 1196 base pairs long, contains a long open reading frame which could code for a protein of 317 amino acids, and has imperfect terminal inverted repeats of 6 base pairs (confirmed by the terminal sequencing of 4.5 copies of IS1000), but does not cause a target site duplication. There are at least 6 copies of IS1000 in the genome ofT. thermophilus HB8. A search of the GEN-EMBL data base revealed that the putative 317 ammo acid protein had significant homology with open reading frames in the transposable elements IS110 ofStreptomyces coelicolor and IS492 ofPseudomonas atlantica.

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U2 - 10.1016/0147-619X(90)90020-D

DO - 10.1016/0147-619X(90)90020-D

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C2 - 2176725

AN - SCOPUS:0025134658

SN - 0147-619X

VL - 24

SP - 1

EP - 11

JO - Plasmid

JF - Plasmid

IS - 1

ER -

Cloning and sequence of IS1000, a putative insertion sequence fromThermus thermophilus HB8

Abstract

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