Cloning, expression and functional characterization of in-house prepared human basic fibroblast growth factor

Hassan Rassouli, Mohammad Sharif Tabe Bordbar, Mehran Rezaei Larijani, Mohammad Pakzad, Hossein Baharvand*, Ghasem Hosseini Salekdeh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Objective: Human basic fibroblast growth factor (bFGF) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bFGF is an essential growth factor for the maintenance of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Materials and Methods: In this experimental study, we present a straightforward method to produce biologically active recombinant human bFGF protein in E. coli that has long-term storage ability. Results: This procedure provides a rapid, cost effective purification of a soluble human bFGF protein that is biologically active and functional as measured in hESCs and hiPSCs in vitro and in vivo. Conclusion: The results show no significant difference in function between our in-house produced and commercialized bFGF.

Original languageEnglish
Pages (from-to)282-291
Number of pages10
JournalCell Journal
Volume14
Issue number4
Publication statusPublished - 1 Dec 2013
Externally publishedYes

Keywords

  • Basic fibroblast growth factor (bFGF)
  • Cell proliferation
  • Embryonic stem cells
  • Pluripotency
  • Recombinant protein

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