Cloning, expression and functional characterization of in-house prepared human basic fibroblast growth factor

Hassan Rassouli; Mohammad Sharif Tabe Bordbar; Mehran Rezaei Larijani; Mohammad Pakzad; Hossein Baharvand; Ghasem Hosseini Salekdeh

Cloning, expression and functional characterization of in-house prepared human basic fibroblast growth factor

Hassan Rassouli, Mohammad Sharif Tabe Bordbar, Mehran Rezaei Larijani, Mohammad Pakzad, Hossein Baharvand^*, Ghasem Hosseini Salekdeh

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Objective: Human basic fibroblast growth factor (bFGF) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bFGF is an essential growth factor for the maintenance of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Materials and Methods: In this experimental study, we present a straightforward method to produce biologically active recombinant human bFGF protein in E. coli that has long-term storage ability. Results: This procedure provides a rapid, cost effective purification of a soluble human bFGF protein that is biologically active and functional as measured in hESCs and hiPSCs in vitro and in vivo. Conclusion: The results show no significant difference in function between our in-house produced and commercialized bFGF.

Original language	English
Pages (from-to)	282-291
Number of pages	10
Journal	Cell Journal
Volume	14
Issue number	4
Publication status	Published - 1 Dec 2013
Externally published	Yes

Keywords

Basic fibroblast growth factor (bFGF)
Cell proliferation
Embryonic stem cells
Pluripotency
Recombinant protein

Cite this

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title = "Cloning, expression and functional characterization of in-house prepared human basic fibroblast growth factor",

abstract = "Objective: Human basic fibroblast growth factor (bFGF) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bFGF is an essential growth factor for the maintenance of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Materials and Methods: In this experimental study, we present a straightforward method to produce biologically active recombinant human bFGF protein in E. coli that has long-term storage ability. Results: This procedure provides a rapid, cost effective purification of a soluble human bFGF protein that is biologically active and functional as measured in hESCs and hiPSCs in vitro and in vivo. Conclusion: The results show no significant difference in function between our in-house produced and commercialized bFGF.",

keywords = "Basic fibroblast growth factor (bFGF), Cell proliferation, Embryonic stem cells, Pluripotency, Recombinant protein",

author = "Hassan Rassouli and Bordbar, {Mohammad Sharif Tabe} and Larijani, {Mehran Rezaei} and Mohammad Pakzad and Hossein Baharvand and Salekdeh, {Ghasem Hosseini}",

year = "2013",

month = dec,

day = "1",

language = "English",

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pages = "282--291",

journal = "Cell Journal",

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TY - JOUR

T1 - Cloning, expression and functional characterization of in-house prepared human basic fibroblast growth factor

AU - Rassouli, Hassan

AU - Bordbar, Mohammad Sharif Tabe

AU - Larijani, Mehran Rezaei

AU - Pakzad, Mohammad

AU - Baharvand, Hossein

AU - Salekdeh, Ghasem Hosseini

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Objective: Human basic fibroblast growth factor (bFGF) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bFGF is an essential growth factor for the maintenance of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Materials and Methods: In this experimental study, we present a straightforward method to produce biologically active recombinant human bFGF protein in E. coli that has long-term storage ability. Results: This procedure provides a rapid, cost effective purification of a soluble human bFGF protein that is biologically active and functional as measured in hESCs and hiPSCs in vitro and in vivo. Conclusion: The results show no significant difference in function between our in-house produced and commercialized bFGF.

AB - Objective: Human basic fibroblast growth factor (bFGF) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bFGF is an essential growth factor for the maintenance of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Materials and Methods: In this experimental study, we present a straightforward method to produce biologically active recombinant human bFGF protein in E. coli that has long-term storage ability. Results: This procedure provides a rapid, cost effective purification of a soluble human bFGF protein that is biologically active and functional as measured in hESCs and hiPSCs in vitro and in vivo. Conclusion: The results show no significant difference in function between our in-house produced and commercialized bFGF.

KW - Basic fibroblast growth factor (bFGF)

KW - Cell proliferation

KW - Embryonic stem cells

KW - Pluripotency

KW - Recombinant protein

UR - http://www.scopus.com/inward/record.url?scp=84874365902&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84874365902

SN - 2228-5806

VL - 14

SP - 282

EP - 291

JO - Cell Journal

JF - Cell Journal

IS - 4

ER -

Cloning, expression and functional characterization of in-house prepared human basic fibroblast growth factor

Abstract

Keywords

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