Cloning of Aspergillus niger genes in yeast. Expression of the gene coding Aspergillusβ-glucosidase

Meria E. Penttilä; K. M. Helena Nevalainen; Alain Raynal; Jonathan K C Knowles

doi:10.1007/BF00425564

Cloning of Aspergillus niger genes in yeast. Expression of the gene coding Aspergillusβ-glucosidase

Meria E. Penttilä, K. M. Helena Nevalainen, Alain Raynal, Jonathan K C Knowles^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.

Original language	English
Pages (from-to)	494-499
Number of pages	6
Journal	MGG Molecular & General Genetics
Volume	194
Issue number	3
DOIs	https://doi.org/10.1007/BF00425564
Publication status	Published - May 1984
Externally published	Yes

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abstract = "The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.",

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AU - Raynal, Alain

AU - Knowles, Jonathan K C

PY - 1984/5

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AB - The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.

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Cloning of Aspergillus niger genes in yeast. Expression of the gene coding Aspergillusβ-glucosidase

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