Cloning of Aspergillus niger genes in yeast. Expression of the gene coding Aspergillusβ-glucosidase

Meria E. Penttilä, K. M. Helena Nevalainen, Alain Raynal, Jonathan K C Knowles*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.

Original languageEnglish
Pages (from-to)494-499
Number of pages6
JournalMGG Molecular & General Genetics
Volume194
Issue number3
DOIs
Publication statusPublished - May 1984
Externally publishedYes

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