Cloning, sequencing and enhanced expression of the Trichoderma reesei endoxylanase II (pI 9) gene xln2

Ritva Saarelainen*, Marja Paloheimo, Richard Fagerström, Pirkko L. Suominen, K. M Helena Nevalainen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

The Trichoderma reesei xln2 gene coding for the pI 9.0 endoxylanase was isolated from the wild-type strain QM6a. The gene contains one intron of 108 nucleotides and codes for a protein of 223 amino acids in which two putative N-glycosylation target sites were found. Three different T. reesei strains were transformed by targeting a construct composed of the xln2 gene, including its promoter, to the endogenous cbh1 locus. Highest overall production levels of xylanase were obtained using T. reesei ALK02721, a genetically engineered strain, as a host. Integration into the cbh1 locus was not required for enhanced expression under control of the xln2 promoter.

Original languageEnglish
Pages (from-to)497-503
Number of pages7
JournalMGG Molecular & General Genetics
Volume241
Issue number5-6
DOIs
Publication statusPublished - Dec 1993
Externally publishedYes

Keywords

  • Expression
  • Gene sequence
  • Trichoderma reesei
  • Xylanase

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