Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection

B. C. Ferrari; M. L. Power; P. L. Bergquist

doi:10.1007/s10529-007-9487-2

Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection

B. C. Ferrari^*, M. L. Power, P. L. Bergquist

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.

Original language	English
Pages (from-to)	1831-1837
Number of pages	7
Journal	Biotechnology Letters
Volume	29
Issue number	12
DOIs	https://doi.org/10.1007/s10529-007-9487-2
Publication status	Published - Dec 2007

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abstract = "Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.",

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AU - Bergquist, P. L.

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AB - Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.

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Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection

Abstract

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