Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection

B. C. Ferrari, M. L. Power, P. L. Bergquist

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.

LanguageEnglish
Pages1831-1837
Number of pages7
JournalBiotechnology Letters
Volume29
Issue number12
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Parasites
Contamination
DNA
Peptide Hydrolases
Apicomplexa
Amplification
Nematoda
Automation
Personnel

Cite this

@article{0d783939b95046f0af9008b4745ce9b5,
title = "Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection",
abstract = "Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.",
author = "Ferrari, {B. C.} and Power, {M. L.} and Bergquist, {P. L.}",
year = "2007",
month = "12",
doi = "10.1007/s10529-007-9487-2",
language = "English",
volume = "29",
pages = "1831--1837",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer, Springer Nature",
number = "12",

}

Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection. / Ferrari, B. C.; Power, M. L.; Bergquist, P. L.

In: Biotechnology Letters, Vol. 29, No. 12, 12.2007, p. 1831-1837.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection

AU - Ferrari, B. C.

AU - Power, M. L.

AU - Bergquist, P. L.

PY - 2007/12

Y1 - 2007/12

N2 - Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.

AB - Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.

UR - http://www.scopus.com/inward/record.url?scp=35748977394&partnerID=8YFLogxK

U2 - 10.1007/s10529-007-9487-2

DO - 10.1007/s10529-007-9487-2

M3 - Article

VL - 29

SP - 1831

EP - 1837

JO - Biotechnology Letters

T2 - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 12

ER -