Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection

B. C. Ferrari*, M. L. Power, P. L. Bergquist

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation.

Original languageEnglish
Pages (from-to)1831-1837
Number of pages7
JournalBiotechnology Letters
Volume29
Issue number12
DOIs
Publication statusPublished - Dec 2007

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