Cold adaptation in the marine bacterium, Sphingopyxis alaskensis, assessed using quantitative proteomics

Lily Ting, Timothy J. Williams, Mark J. Cowley, Federico M. Lauro, Michael Guilhaus, Mark J. Raftery, Ricardo Cavicchioli*

*Corresponding author for this work

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The cold marine environment constitutes a large proportion of the Earth's biosphere. Sphingopyxis alaskensis was isolated as a numerically abundant bacterium from several cold marine locations, and has been extensively studied as a model marine bacterium. Recently, a metabolic labelling platform was developed to comprehensively identify and quantify proteins from S. alaskensis. The approach incorporated data normalization and statistical validation for the purpose of generating highly confident quantitative proteomics data. Using this approach, we determined quantitative differences between cells grown at 10°C (low temperature) and 30°C (high temperature). Cold adaptation was linked to specific aspects of gene expression: a dedicated protein-folding system using GroESL, DnaK, DnaJ, GrpE, SecB, ClpB and PPIase; polyhydroxyalkanoate-associated storage materials; a link between enzymes in fatty acid metabolism and energy generation; de novo synthesis of polyunsaturated fatty acids in the membrane and cell wall; inorganic phosphate ion transport by a phosphate import PstB homologue; TonB-dependent receptor and bacterioferritin in iron homeostasis; histidine, tryptophan and proline amino acid metabolism; and a large number of proteins without annotated functions. This study provides a new level of understanding on how important marine bacteria can adapt to compete effectively in cold marine environments. This study is also a benchmark for comparative proteomic analyses with other important marine bacteria and other cold-adapted organisms.

Original languageEnglish
Pages (from-to)2658-2676
Number of pages19
JournalEnvironmental microbiology
Volume12
Issue number10
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

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    Ting, L., Williams, T. J., Cowley, M. J., Lauro, F. M., Guilhaus, M., Raftery, M. J., & Cavicchioli, R. (2010). Cold adaptation in the marine bacterium, Sphingopyxis alaskensis, assessed using quantitative proteomics. Environmental microbiology, 12(10), 2658-2676. https://doi.org/10.1111/j.1462-2920.2010.02235.x