Combined antibody/lectin-enrichment identifies extensive changes in the O-GlcNAc subproteome upon oxidative stress

Albert Lee; Natasha E. Zachara

Combined antibody/lectin-enrichment identifies extensive changes in the O-GlcNAc subproteome upon oxidative stress

Albert Lee, Natasha E. Zachara

Research output: Non-traditional research output › Data set/Database

Abstract

O-GlcNAc is thought to regulate proteins in a mannaer analogous to other PTMs, modulating cellular functions including the cellular stress response. The aim of this study was to identify specific cellular networks and protein complexes that are differentially O-GlcNAcylated and/or expressed upon acute oxidative stress (H2O2 for 1 and 2 h). We achieved this by employing SILAC and a novel anti-O-GlcNAc G5 lectibody IP strategy that combind O-GlcNAc specific antibodies and the lectin WGA. We identified 990 proteins among all three treatments including 202 non-redundant O-GlcNAc modified peptides. Differentially expressed proteins clustered into canonical 14-3-3/PI3K signaling pathways including the 14-3-3 complex, chaperonins, RNA Pol II Mediator and nuclear pore complexes.

Original language	English
Publication status	Published - 29 Sept 2016

Cite this

@misc{6366771207c04aab838ec884eaa0c4df,

title = "Combined antibody/lectin-enrichment identifies extensive changes in the O-GlcNAc subproteome upon oxidative stress",

abstract = "O-GlcNAc is thought to regulate proteins in a mannaer analogous to other PTMs, modulating cellular functions including the cellular stress response. The aim of this study was to identify specific cellular networks and protein complexes that are differentially O-GlcNAcylated and/or expressed upon acute oxidative stress (H2O2 for 1 and 2 h). We achieved this by employing SILAC and a novel anti-O-GlcNAc G5 lectibody IP strategy that combind O-GlcNAc specific antibodies and the lectin WGA. We identified 990 proteins among all three treatments including 202 non-redundant O-GlcNAc modified peptides. Differentially expressed proteins clustered into canonical 14-3-3/PI3K signaling pathways including the 14-3-3 complex, chaperonins, RNA Pol II Mediator and nuclear pore complexes.",

author = "Albert Lee and Zachara, {Natasha E.}",

year = "2016",

month = sep,

day = "29",

language = "English",

}

TY - ADVS

T1 - Combined antibody/lectin-enrichment identifies extensive changes in the O-GlcNAc subproteome upon oxidative stress

AU - Lee, Albert

AU - Zachara, Natasha E.

PY - 2016/9/29

Y1 - 2016/9/29

N2 - O-GlcNAc is thought to regulate proteins in a mannaer analogous to other PTMs, modulating cellular functions including the cellular stress response. The aim of this study was to identify specific cellular networks and protein complexes that are differentially O-GlcNAcylated and/or expressed upon acute oxidative stress (H2O2 for 1 and 2 h). We achieved this by employing SILAC and a novel anti-O-GlcNAc G5 lectibody IP strategy that combind O-GlcNAc specific antibodies and the lectin WGA. We identified 990 proteins among all three treatments including 202 non-redundant O-GlcNAc modified peptides. Differentially expressed proteins clustered into canonical 14-3-3/PI3K signaling pathways including the 14-3-3 complex, chaperonins, RNA Pol II Mediator and nuclear pore complexes.

AB - O-GlcNAc is thought to regulate proteins in a mannaer analogous to other PTMs, modulating cellular functions including the cellular stress response. The aim of this study was to identify specific cellular networks and protein complexes that are differentially O-GlcNAcylated and/or expressed upon acute oxidative stress (H2O2 for 1 and 2 h). We achieved this by employing SILAC and a novel anti-O-GlcNAc G5 lectibody IP strategy that combind O-GlcNAc specific antibodies and the lectin WGA. We identified 990 proteins among all three treatments including 202 non-redundant O-GlcNAc modified peptides. Differentially expressed proteins clustered into canonical 14-3-3/PI3K signaling pathways including the 14-3-3 complex, chaperonins, RNA Pol II Mediator and nuclear pore complexes.

UR - https://www.ebi.ac.uk/pride/archive/projects/PXD000849

M3 - Data set/Database

ER -

Combined antibody/lectin-enrichment identifies extensive changes in the O-GlcNAc subproteome upon oxidative stress

Abstract

Other files and links

Fingerprint

Combined Antibody/Lectin enrichment identifies extensive changes in the O-GIcNAc Sub-proteome upon Oxidative stress

Cite this