Combined in situ hybridization and immunohistochemistry in rat brain tissue using digoxigenin-labeled riboprobes

Natasha N. Kumar; Belinda R. Bowman; Ann K. Goodchild

doi:10.1007/978-1-61779-897-9_2

Combined in situ hybridization and immunohistochemistry in rat brain tissue using digoxigenin-labeled riboprobes

Natasha N. Kumar, Belinda R. Bowman, Ann K. Goodchild

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

This protocol describes a procedure for combining non-radioactive in situ hybridization (ISH) histochemistry with multi-label fluorescence immunohistochemistry (IHC) on rat brain tissue sections. This allows visualization of multiple mRNA and protein targets located within the same or different subcellular compartments. A comprehensive description of RNA probe preparation, validation and storage conditions is described. This is followed by the tissue preparation and tissue processing procedure for combined ISH/IHC on free-floating brain sections. Both the tissue and the RNA probes must be prepared and handle under strict RNase-free conditions up until the point of RNA hybridization.

Original language	English
Pages (from-to)	31-52
Number of pages	22
Journal	Neuromethods
Volume	70
DOIs	https://doi.org/10.1007/978-1-61779-897-9_2
Publication status	Published - 2012

Access to Document

10.1007/978-1-61779-897-9_2

Cite this

@article{5d4e05d0a21449f0955562f7dfc62489,

title = "Combined in situ hybridization and immunohistochemistry in rat brain tissue using digoxigenin-labeled riboprobes",

abstract = "This protocol describes a procedure for combining non-radioactive in situ hybridization (ISH) histochemistry with multi-label fluorescence immunohistochemistry (IHC) on rat brain tissue sections. This allows visualization of multiple mRNA and protein targets located within the same or different subcellular compartments. A comprehensive description of RNA probe preparation, validation and storage conditions is described. This is followed by the tissue preparation and tissue processing procedure for combined ISH/IHC on free-floating brain sections. Both the tissue and the RNA probes must be prepared and handle under strict RNase-free conditions up until the point of RNA hybridization.",

author = "Kumar, {Natasha N.} and Bowman, {Belinda R.} and Goodchild, {Ann K.}",

year = "2012",

doi = "10.1007/978-1-61779-897-9_2",

language = "English",

volume = "70",

pages = "31--52",

journal = "Neuromethods",

issn = "0893-2336",

publisher = "Springer, Springer Nature",

}

TY - JOUR

T1 - Combined in situ hybridization and immunohistochemistry in rat brain tissue using digoxigenin-labeled riboprobes

AU - Kumar, Natasha N.

AU - Bowman, Belinda R.

AU - Goodchild, Ann K.

PY - 2012

Y1 - 2012

N2 - This protocol describes a procedure for combining non-radioactive in situ hybridization (ISH) histochemistry with multi-label fluorescence immunohistochemistry (IHC) on rat brain tissue sections. This allows visualization of multiple mRNA and protein targets located within the same or different subcellular compartments. A comprehensive description of RNA probe preparation, validation and storage conditions is described. This is followed by the tissue preparation and tissue processing procedure for combined ISH/IHC on free-floating brain sections. Both the tissue and the RNA probes must be prepared and handle under strict RNase-free conditions up until the point of RNA hybridization.

AB - This protocol describes a procedure for combining non-radioactive in situ hybridization (ISH) histochemistry with multi-label fluorescence immunohistochemistry (IHC) on rat brain tissue sections. This allows visualization of multiple mRNA and protein targets located within the same or different subcellular compartments. A comprehensive description of RNA probe preparation, validation and storage conditions is described. This is followed by the tissue preparation and tissue processing procedure for combined ISH/IHC on free-floating brain sections. Both the tissue and the RNA probes must be prepared and handle under strict RNase-free conditions up until the point of RNA hybridization.

U2 - 10.1007/978-1-61779-897-9_2

DO - 10.1007/978-1-61779-897-9_2

M3 - Article

SN - 0893-2336

VL - 70

SP - 31

EP - 52

JO - Neuromethods

JF - Neuromethods

ER -

Combined in situ hybridization and immunohistochemistry in rat brain tissue using digoxigenin-labeled riboprobes

Abstract

Access to Document

Fingerprint

Cite this