Combined N-glycome and N-glycoproteome analysis of the lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes

Svend Dam, Morten Thaysen-Andersen, Eva Stenkjær, Andrea Lorentzen, Peter Roepstorff, Nicolle H. Packer, Jens Stougaard*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    Legume food allergy, such as allergy toward peanuts and soybeans, is a health issue predicted to worsen as dietary advice recommends higher intake of legume-based foods. Lotus japonicus (Lotus) is an established legume plant model system for studies of symbiotic and pathogenic microbial interactions and, due to its well characterized genotype/phenotype and easily manipulated genome, may also be suitable for studies of legume food allergy. Here we present a comprehensive study of the Lotus N-glycoproteome. The global and site-specific N-glycan structures of Lotus seed globulins were analyzed using mass spectrometry-based glycomics and glycoproteomics techniques. In total, 19 N-glycan structures comprising high mannose (∼20%), pauci-mannosidic (∼40%), and complex forms (∼40%) were determined. The pauci-mannosidic and complex N-glycans contained high amounts of the typical plant determinants β-1,2-xylose and α-1,3-fucose. Two abundant Lotus seed N-glycoproteins were site-specifically profiled; a predicted lectin containing two fully occupied N-glycosylation sites carried predominantly pauci-mannosidic structures in different distributions. In contrast, Lotus convicilin storage protein 2 (LCP2) carried exclusively high mannose N-glycans similar to its homologue, Ara h 1, which is the major allergen in peanut. In silico investigation confirmed that peanut Ara h 1 and Lotus LCP2 are highly similar at the primary and higher protein structure levels. Hence, we suggest that Lotus has the potential to serve as a model system for studying the role of seed proteins and their glycosylation in food allergy.

    Original languageEnglish
    Pages (from-to)3383-3392
    Number of pages10
    JournalJournal of Proteome Research
    Volume12
    Issue number7
    DOIs
    Publication statusPublished - 5 Jul 2013

    Keywords

    • Ara h 1
    • food allergy
    • globulin
    • glycomics
    • glycoproteomics
    • Lotus japonicus
    • N-glycosylation
    • peanut

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