Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity

Michael Stat, Jeffrey John, Joseph D. DiBattista, Stephen J. Newman, Michael Bunce, Euan S. Harvey

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Monitoring communities of fish is important for the management and sustainability of fisheries and marine ecosystems. Baited remote underwater video systems (BRUVs) are among the most effective nondestructive techniques for sampling bony fishes and elasmobranchs (sharks, rays, and skates). However, BRUVs sample visually conspicuous biota; hence, some taxa are undersampled or not recorded at all. We compared the diversity of fishes characterized using BRUVs with diversity detected via environmental DNA (eDNA) metabarcoding. We sampled seawater and captured BRUVs imagery at 48 locales that included reef and seagrass beds inside and outside a marine reserve (Jurien Bay in Western Australia). Eighty-two fish genera from 13 orders were detected, and the community of fishes described using eDNA and BRUVs combined yielded >30% more generic richness than when either method was used alone. Rather than detecting a homogenous genetic signature, the eDNA assemblages mirrored the BRUVs’ spatial explicitness; differentiation of taxa between seagrass and reef was clear despite the relatively small geographical scale of the study site (∼35 km2). Taxa that were not sampled by one approach, due to limitations and biases intrinsic to the method, were often detected with the other. Therefore, using BRUVs and eDNA in concert provides a more holistic view of vertebrate marine communities across habitats. Both methods are noninvasive, which enhances their potential for widespread implementation in the surveillance of marine ecosystems.

LanguageEnglish
Pages196-205
Number of pages10
JournalConservation Biology
Volume33
Issue number1
Early online date13 Jul 2018
DOIs
Publication statusPublished - Feb 2019

Fingerprint

biodiversity
DNA
monitoring
fish
reefs
Rajidae
seagrass
marine ecosystem
methodology
sharks
Western Australia
reef
seawater
vertebrates
fisheries
marine park
sampling
shark
video
surveillance

Bibliographical note

Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • baited remote underwater video systems
  • elasmobranchs
  • environmental DNA
  • environmental genomics
  • marine management
  • ADN ambiental
  • elasmobranquios
  • genomica ambiental
  • manejo marino
  • sistemas remotos de video submarino con carnada

Cite this

Stat, M., John, J., DiBattista, J. D., Newman, S. J., Bunce, M., & Harvey, E. S. (2019). Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity. Conservation Biology, 33(1), 196-205. https://doi.org/10.1111/cobi.13183
Stat, Michael ; John, Jeffrey ; DiBattista, Joseph D. ; Newman, Stephen J. ; Bunce, Michael ; Harvey, Euan S. / Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity. In: Conservation Biology. 2019 ; Vol. 33, No. 1. pp. 196-205.
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Stat, M, John, J, DiBattista, JD, Newman, SJ, Bunce, M & Harvey, ES 2019, 'Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity' Conservation Biology, vol. 33, no. 1, pp. 196-205. https://doi.org/10.1111/cobi.13183

Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity. / Stat, Michael; John, Jeffrey; DiBattista, Joseph D.; Newman, Stephen J.; Bunce, Michael; Harvey, Euan S.

In: Conservation Biology, Vol. 33, No. 1, 02.2019, p. 196-205.

Research output: Contribution to journalArticleResearchpeer-review

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