Comparative analysis of the protein profile from biofortified cultivars of quality protein maize and conventional maize by gel-based and gel-free proteomic approaches

Priscila R. dos Santos-Donado; Carlos M. Donado-Pestana; Rebeca Kawahara; Livia Rosa-Fernandes; Giuseppe Palmisano; Flavio Finardi-Filho

doi:10.1016/j.lwt.2020.110683

Comparative analysis of the protein profile from biofortified cultivars of quality protein maize and conventional maize by gel-based and gel-free proteomic approaches

Priscila R. dos Santos-Donado, Carlos M. Donado-Pestana, Rebeca Kawahara, Livia Rosa-Fernandes, Giuseppe Palmisano, Flavio Finardi-Filho

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Abstract

Biofortification of crops has promoted the development of quality protein maize (QPM), which are rich in essential amino acids. However, the proteomic rebalancing of QPM cultivars grown in Brazil remains unknown. Here, we show the compositional equivalence and protein profiles from two QPM cultivars (BR-473 and BR-451, named here as QPM1 and QPM2, respectively) compared to a commercial non-QPM hybrid (BRS-4103, non-QPM), by using complementary 2-DE-based (gel-based) and bottom-up shotgun (gel-free) proteomic approaches. The compositional equivalence observed in these cultivars was expected for Zea mays. Among differentially expressed proteins (DEPs) identified, storage proteins as non-zeins were generally up-regulated, where zeins were down-regulated in QPM cultivars. In turn, defense and cell stress proteins as heat shock protein (HSP) and ribosome-inactivating protein (RIP) were down-regulated. Together, these findings point the superior nutritional value of proteins from QPM cultivars as well as their susceptibility to pathogens.

Original language	English
Article number	110683
Pages (from-to)	1-20
Number of pages	20
Journal	LWT
Volume	138
DOIs	https://doi.org/10.1016/j.lwt.2020.110683
Publication status	Published - Mar 2021

Bibliographical note

Copyright the Publisher 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Zea mays L.
Biofortification
Mass spectrometry
Amino acids

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10.1016/j.lwt.2020.110683Licence: CC BY-NC-ND

Publisher version (open access)Final published version, 4.52 MBLicence: CC BY-NC-ND

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title = "Comparative analysis of the protein profile from biofortified cultivars of quality protein maize and conventional maize by gel-based and gel-free proteomic approaches",

abstract = "Biofortification of crops has promoted the development of quality protein maize (QPM), which are rich in essential amino acids. However, the proteomic rebalancing of QPM cultivars grown in Brazil remains unknown. Here, we show the compositional equivalence and protein profiles from two QPM cultivars (BR-473 and BR-451, named here as QPM1 and QPM2, respectively) compared to a commercial non-QPM hybrid (BRS-4103, non-QPM), by using complementary 2-DE-based (gel-based) and bottom-up shotgun (gel-free) proteomic approaches. The compositional equivalence observed in these cultivars was expected for Zea mays. Among differentially expressed proteins (DEPs) identified, storage proteins as non-zeins were generally up-regulated, where zeins were down-regulated in QPM cultivars. In turn, defense and cell stress proteins as heat shock protein (HSP) and ribosome-inactivating protein (RIP) were down-regulated. Together, these findings point the superior nutritional value of proteins from QPM cultivars as well as their susceptibility to pathogens.",

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AU - Santos-Donado, Priscila R. dos

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AU - Kawahara, Rebeca

AU - Rosa-Fernandes, Livia

AU - Palmisano, Giuseppe

AU - Finardi-Filho, Flavio

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Comparative analysis of the protein profile from biofortified cultivars of quality protein maize and conventional maize by gel-based and gel-free proteomic approaches

Abstract

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Keywords

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