TY - JOUR
T1 - Comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance
AU - Faghani, Elham
AU - Gharechahi, Javad
AU - Komatsu, Setsuko
AU - Mirzaei, Mehdi
AU - Khavarinejad, Ramzan Ali
AU - Najafi, Farzaneh
AU - Farsad, Laleh Karimi
AU - Salekdeh, Ghasem Hosseini
PY - 2015/1/30
Y1 - 2015/1/30
N2 - Comparative physiology and proteomic analyses were conducted to monitor the stress response of two wheat genotypes (SERI M 82 (SE) and SW89.5193/kAu2 (SW)) with contrasting responses to drought stress. Under stress condition, the tolerant genotype (SE) produced higher shoot and root biomasses, longer roots and accumulated higher level of ABA in leaves. Physiological measurements suggested that the SE genotype was more efficient in water absorption and could preserve more water presumably by controlling stomata closure. Proteomic analysis showed an increased abundance of proteins related to defense and oxidative stress responses such as GLPs, GST, and SOD, and those related to protein processing such as small HSPs in roots of both genotypes in response to drought stress. Interestingly, the abundance of proteins such as endo-1,3-beta-glucosidase, peroxidase, SAMS, and MDH significantly increased in roots or leaves of the SE genotype and decreased in that of the SW one. In addition, an increased abundance of APX was detected in leaves and roots of the SE genotype and a decreased abundance of 14-3-3 and ribosomal proteins were noted in the SW one in response to drought stress. Our findings led to a better understanding about the integrated physiology and proteome responses of wheat genotypes with nearly contrasting responses to drought stress.
AB - Comparative physiology and proteomic analyses were conducted to monitor the stress response of two wheat genotypes (SERI M 82 (SE) and SW89.5193/kAu2 (SW)) with contrasting responses to drought stress. Under stress condition, the tolerant genotype (SE) produced higher shoot and root biomasses, longer roots and accumulated higher level of ABA in leaves. Physiological measurements suggested that the SE genotype was more efficient in water absorption and could preserve more water presumably by controlling stomata closure. Proteomic analysis showed an increased abundance of proteins related to defense and oxidative stress responses such as GLPs, GST, and SOD, and those related to protein processing such as small HSPs in roots of both genotypes in response to drought stress. Interestingly, the abundance of proteins such as endo-1,3-beta-glucosidase, peroxidase, SAMS, and MDH significantly increased in roots or leaves of the SE genotype and decreased in that of the SW one. In addition, an increased abundance of APX was detected in leaves and roots of the SE genotype and a decreased abundance of 14-3-3 and ribosomal proteins were noted in the SW one in response to drought stress. Our findings led to a better understanding about the integrated physiology and proteome responses of wheat genotypes with nearly contrasting responses to drought stress.
KW - Wheat
KW - Drought stress
KW - Relative water content
KW - Two-dimensional gel electrophoresis
KW - Proteomics
KW - Mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=84911890827&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2014.10.018
DO - 10.1016/j.jprot.2014.10.018
M3 - Article
C2 - 25449836
AN - SCOPUS:84911890827
SN - 1874-3919
VL - 114
SP - 1
EP - 15
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -