Comparative proteomic analysis of mouse embryonic stem cells and neonatal-derived cardiomyocytes

Hossein Baharvand*, Mohsen Hajheidari, Roseata Zonouzi, Saeid Kazemi Ashtiani, Saman Hosseinkhani, Ghasem Hosseini Salekdeh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Pluripotent embryonic stem cells (ESCs) spontaneously differentiate via embryo-like aggregates into cardiomyocytes. A thorough understanding of the molecular conditions in ESCs is necessary before other potential applications of these cells such as cell therapy can be materialized. We applied two dimensional electrophoresis to analyze and compare the proteome profiling of spontaneous mouse ESC-derived cardiomyocytes (ESC-DCs), undifferentiated mouse ESCs, and neonatal-derived cardiomyocytes (N-DCs). Ninety-five percent of the proteins detected on the ESC-DCs and N-DCs could be precisely paired with one other, whereas only twenty percent of the ESC proteins could be reliably matched with those on the ESC-DCs and N-DCSs, suggesting a striking similarity between them. Having identified sixty proteins in the said three cell types, we sought to provide possible explanations for their differential expression patterns and discuss their relevance to cell biology. This study provides a new insight into the gene expression pattern of differentiated cardiomyocytes and is further evidence for a close relation between ESC-DCs and N-DCSs.

Original languageEnglish
Pages (from-to)1041-1049
Number of pages9
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 27 Oct 2006
Externally publishedYes


  • Cardiomyocytes
  • Differentiation
  • Embryonic stem cells
  • Proteomics


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