Comparison between different sheathless electrospray emitter configurations regarding the performance of nanoscale liquid chromatography-time-of-flight mass spectrometry analysis

Ardeshir Amirkhani; Magnus Wetterhall; Stefan Nilsson; Rolf Danielsson; Jonas Bergquist

doi:10.1016/j.chroma.2004.01.063

Comparison between different sheathless electrospray emitter configurations regarding the performance of nanoscale liquid chromatography-time-of-flight mass spectrometry analysis

Ardeshir Amirkhani, Magnus Wetterhall, Stefan Nilsson, Rolf Danielsson, Jonas Bergquist^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Four different sheathless electrospray ionization (ESI) configurations were investigated for a nano liquid chromatography (LC) system. The studied configurations were: a column with an integrated emitter, with the ESI potential applied before or after the column, and a column with separate emitter, with the ESI voltage applied at a union before the emitter or at the emitter tip. The results indicates that the efficiency of the LC system is rather independent of the configuration when using 95 μm i.d. columns, acetic mobile phase and standard peptides as a sample. Introduction of post column dead volume seems not to be a critical issue at least with flow rates down to 600 nl/min.

Original language	English
Pages (from-to)	257-266
Number of pages	10
Journal	Journal of Chromatography A
Volume	1033
Issue number	2
Early online date	2 Mar 2004
DOIs	https://doi.org/10.1016/j.chroma.2004.01.063
Publication status	Published - 16 Apr 2004
Externally published	Yes

Keywords

Nanotechnology
Peptide Mapping
Serum Albumin, Bovine/chemistry
Spectrometry, Mass, Electrospray Ionization/methods
Trypsin/chemistry

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10.1016/j.chroma.2004.01.063

Cite this

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title = "Comparison between different sheathless electrospray emitter configurations regarding the performance of nanoscale liquid chromatography-time-of-flight mass spectrometry analysis",

abstract = "Four different sheathless electrospray ionization (ESI) configurations were investigated for a nano liquid chromatography (LC) system. The studied configurations were: a column with an integrated emitter, with the ESI potential applied before or after the column, and a column with separate emitter, with the ESI voltage applied at a union before the emitter or at the emitter tip. The results indicates that the efficiency of the LC system is rather independent of the configuration when using 95 μm i.d. columns, acetic mobile phase and standard peptides as a sample. Introduction of post column dead volume seems not to be a critical issue at least with flow rates down to 600 nl/min.",

keywords = "Nanotechnology, Peptide Mapping, Serum Albumin, Bovine/chemistry, Spectrometry, Mass, Electrospray Ionization/methods, Trypsin/chemistry",

author = "Ardeshir Amirkhani and Magnus Wetterhall and Stefan Nilsson and Rolf Danielsson and Jonas Bergquist",

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journal = "Journal of Chromatography A",

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Comparison between different sheathless electrospray emitter configurations regarding the performance of nanoscale liquid chromatography-time-of-flight mass spectrometry analysis. / Amirkhani, Ardeshir; Wetterhall, Magnus; Nilsson, Stefan et al.
In: Journal of Chromatography A, Vol. 1033, No. 2, 16.04.2004, p. 257-266.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Comparison between different sheathless electrospray emitter configurations regarding the performance of nanoscale liquid chromatography-time-of-flight mass spectrometry analysis

AU - Amirkhani, Ardeshir

AU - Wetterhall, Magnus

AU - Nilsson, Stefan

AU - Danielsson, Rolf

AU - Bergquist, Jonas

PY - 2004/4/16

Y1 - 2004/4/16

N2 - Four different sheathless electrospray ionization (ESI) configurations were investigated for a nano liquid chromatography (LC) system. The studied configurations were: a column with an integrated emitter, with the ESI potential applied before or after the column, and a column with separate emitter, with the ESI voltage applied at a union before the emitter or at the emitter tip. The results indicates that the efficiency of the LC system is rather independent of the configuration when using 95 μm i.d. columns, acetic mobile phase and standard peptides as a sample. Introduction of post column dead volume seems not to be a critical issue at least with flow rates down to 600 nl/min.

AB - Four different sheathless electrospray ionization (ESI) configurations were investigated for a nano liquid chromatography (LC) system. The studied configurations were: a column with an integrated emitter, with the ESI potential applied before or after the column, and a column with separate emitter, with the ESI voltage applied at a union before the emitter or at the emitter tip. The results indicates that the efficiency of the LC system is rather independent of the configuration when using 95 μm i.d. columns, acetic mobile phase and standard peptides as a sample. Introduction of post column dead volume seems not to be a critical issue at least with flow rates down to 600 nl/min.

KW - Nanotechnology

KW - Peptide Mapping

KW - Serum Albumin, Bovine/chemistry

KW - Spectrometry, Mass, Electrospray Ionization/methods

KW - Trypsin/chemistry

UR - https://www.scopus.com/pages/publications/1542298912

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DO - 10.1016/j.chroma.2004.01.063

M3 - Article

C2 - 15088746

SN - 0021-9673

VL - 1033

SP - 257

EP - 266

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 2

ER -

Comparison between different sheathless electrospray emitter configurations regarding the performance of nanoscale liquid chromatography-time-of-flight mass spectrometry analysis

Abstract

Keywords

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