Comparison of PEI-PEG and PLL-PEG copolymer coatings on the prevention of protein fouling

Anna Bergstrand, Ghazal Rahmani-Monfared, Åsa Östlund, Magnus Nydén, Krister Holmberg*

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Citations (Scopus)


The effect of surface charge on the protein resistance of adsorbed layers of poly(ethylene imine)-[g]-poly(ethylene glycol), PEI-PEG, and poly(L-lysine)-[g]-poly(ethylene glycol), PLL-PEG, was studied. Mixed and monofunctional self-assembled monolayers, SAMs, on gold were obtained by adsorption of 16-mercapto-l-hexadeca-noic acid and 16-mercapto-l-hexadecanol. The surface charge was systematically varied by changing the ratio of the two alkanethiols. The graft copolymers PEI-PEG and PLL-PEG were adsorbed at the SAMs and tested for resistance towards human serum albumin and fibrinogen. The adsorbed amount of copolymers increased with increasing negative surface charge. However, the best protein resistance was found at an intermediate surface charge. The PLL-PEG covered surfaces showed better protein resistance than the PEI-PEG covered surfaces. Thus, this work demonstrates that an adsorbed layer of PEG-grafted PEI and, in particular, PEG-grafted PLL is efficient in preventing protein adsorption when there is charge neutralization between the copolymer and the underlying surface.

Original languageEnglish
Pages (from-to)608-615
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Issue number3
Publication statusPublished - 1 Mar 2009
Externally publishedYes


  • poly(ethylene imine)
  • protein rejection
  • selfassembled monolayers
  • poly(L-lysine)
  • poly(ethylene glycol)

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